| Objective:TO investigate the effect of intravenous UTP against the injury following cerebral ischemia/reperfusion(I/R) in rat.Materials and methods:1.Animals:300 male SD rats of 250-350g were used in the studies.2.The middle cerebral artery occlusion(MCAO) by intraluminal suture method was used to reproduce cerebral ischemia.3.TO survey on pharmacodynamics and morphology in focal cerebral ischemia/reperfusion injury:One hundred male SD rats were randomly divided into five groups:sham operation group(A group),I/R group(B group),10ug/kg UTP treatment group(C group),30ug/kg UTP treatment group(D group),90ug/kg UTP treatment group(E group).The perfusion was restored 2 hours after MCAO,and UTP was continuously injected through vena caudalis 0.5 hour after MCAO.After 24 hours of reperfusion,we evaluated the change of praxiology.Eight rats of each group were used to calculate the moisture capacity(MC)of brain tissue.Another Eight rats of each group were used to measure the volume of infarction zone and calculate the percentage of infarction zone on the whole brain tissue.Another four rats of each group were used to survey histomorphology.4.The effects of UTP on the contraction of rat arteria carotis in vitro were examined.Results:The change of praxiology,the moisture capacity(MC)of brain tissue and the volume of infarction zone in C,D and E groups were significantly lower than B group. Pathology showed that UTP can reduce the brain hemorrhage and cellular necrosis,and also can reduce the cerebral edema.The effects of UTP on the contraction of rat arteria carotis in vitro showed that UTP can significantly reduce the blood vessel tension induced by PE.Conclusion:UTP might have significantly protection on focal cerebral ischemia/reperfusion injury rats. |
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