Effects Of Unification Medication Of Glycyrrhizinate And 5-ASA On Expression Of TNF-α, IL-8 And NF-κB In Rats Model With Ulcerative Colitis

Objective To explore mechanism of molecule of glycyrrhizinate and 5-ASA treating ulcerative colitis through observing expression of TNF-α,IL-8 and NF-κB in rats with ulcerative colitis induced by 2,4-dinitro-chlorobenzene and acetic acid.Method 6 rats were taken randomly from 33 rats and become normal control group,27 rats for the rest were induced to have ulcerative colitis by 2,4-dinitro-chlorobenzene and acetic acid. Except for dead rats(3 rats),24 rats for the rest were devided into model control group,5-ASA group,glycyrrhizinate group and unification medication group of 5-ASA and glycyrrhizinate(6 rats in each group) randomly.After that,The rats in normal control group and model control group were given an enema or clyster with normal saline(1ml/200g weight),the rats in 5-ASA group were given an enema or clyster with 5-ASA(1ml/200g weight)(100mg/kg),the rats in glycyrrhizinate group were given an enema or clyster with glycyrrhizinate(1ml/200g weight) (40mg/kg),the rats in unification medication group were given an enema or clyster with 5-ASA(1ml/200g weight)(100mg/kg) and glycyrrhizinate(1ml/200g weight)(40mg/kg),o.d. Symptom of bowel complaint and hemafecia in rats was observed and the change of body weight was recorded every day.After 7 days,blood in the heart of rats were got and blood serum of them were separated from the blood in every group,the level of TNF-α,IL-8 in the blood serum was surveyed and evaluated by using enzyme-linked immunosorbent assay.After that,animals were executed,the colons(8cm) of rats were got,the damage of colonic mucosa in general and the histologic damage were observed,score of colonic damage was determined,and the level of NF-κB in colonic mucosa was determined by immunohistochemistry method.Result 1 In the stage of UC,score of colonic damage advanced obviously,the level of TNF-α,IL-8 in the blood serum and expression of NF-κB in colonic mucosa advanced obviously (P＜0.05),and those were correlated with change of state of the illness(P＜0.05).2 After using glycyrrhizinate or 5-ASA,score of colonic damage in every group degraded obviously,the level of TNF-α,IL-8 in the blood serum and expression of NF-κB in colonic mucosa descended obviously(P＜0.05).3 The effect of glycyrrhizinate lowering the level of TNF-α,IL-8 in the blood serum and expression of NF-κB in colonic mucosa was as good as that of 5-ASA(P＞0.05).4 The effects of unification medication of glycyrrhizinate and 5-ASA lowering the level of TNF-α,IL-8 in the blood serum and expression of NF-κB in colonic mucosa were more excellent than that of single sentero clyster by using 5-aminosalicylic acid or glycyrrhizinate.(P＜0.05).Conclusion Unification utilizing glycyrrhizinate and 5-ASA had a good therapeutic action for rats with ulcerative colitis evoked by 2,4-Dinitro-chlorobenzene and acetic acid.Its mechanism of action is to reduce damage of inflammation by suppressing of expression of TNF-α,IL-8 and NF-κB in rats.