Expression And Significance Of Osteopontin In Serum And Peritoneal Fluid Of Patients With Endometriosis

Objective: To study the expression of osteopontin(OPN)in serum and peritoneal fluid of patients with endometriosis ,and to investigate the role and significance of osteopontin(OPN)on the pathogenesis of endometriosis.Methods: The serum and peritoneal fluid from 30 cases with endometriosis (study group) and 15 cases without endometriosis (control group) that underwent laparoscopic surgery were collected. Patients with endometriosis were grouped according to the Revised American Fertility Society (r-AFS) staging as stageⅠ5 patients , stageⅡ9 patients , stageⅢ13 patients and stageⅣ3 patients. The levels of osteopontin in serum and peritoneal fluid were detected by enzyme-linked immunosorbent assay (ELISA). Data were analyzed according to phase of the menstrual cycle, disease stage, and disease site.Results:1,Comparison of the levels of osteopontin in the serum and the peritoneal fluid(PF) of study group and control group.The levels of OPN in the serum from the study group (27.70±11.77) were higher than that in the serum from the control group (10.71±2.06, P<0.01). The levels of OPN in the PF from the study group (33.43±12.17) were higher than that in the PF from the control group (13.26±1.84, P<0.01).2,Comparison of the levels of osteopontin in the serum and the PF of study group and control group between proliferative stage and secretory stage.There was no obvious difference in the serum of study group between proliferative stage (22.83±8.36) and secretory stage (29.30±11.28,P﹥0.05). The levels of OPN had no relationship with menstrual cycle in the serum of study group. However, there was a significant difference in PF of study group between proliferative stage (27.75±9.35) and secretory stage (35.84±11.19,P<0.05). There was a significant difference in the serum of control group between proliferative stage (8.94±0.76) and secretory stage (12.25±1.46), P<0.01. The levels of OPN had a relationship with menstrual cycle in the serum of control group. However , there was no obvious difference in the PF of control group between proliferative stage ( 12.30±1.25 ) and secretory stage ( 14.10±1.93 ) ,P﹥0.05.3,Comparison of the levels of OPN in the serum and the PF of patients with EMS in mild (Ⅰ,Ⅱ) stage and severe(Ⅲ,Ⅳ) stage.The OPN levels in serum and PF of the patients with severe EMS were significantly higher than those in mild patients (36.70±12.64 vs. 20.82±3.87,P<0.01) (43.83±11.43 vs.25.48±3.92, P<0 .01). Moreover, the OPN levels in serum( r=0.429, P<0.01)and PF (r=0.497,P<0.01) positively correlated with extent of disease.4,The levels of OPN in serum positively correlated with that in PF in both groups ( r=0.956, P<0.01;r=0.765, P<0.01) . However, comparing the OPN levels in serum with that in PF, there was no obvious difference between the two groups(P﹥0.05).5,Comparison of OPN levels in cases with superficial peritoneal EMS and ovarian EMS.The OPN levels in cases with superficial peritoneal EMS(45.57±7.43)were significantly higher than that in cases with ovarian EMS(27.49±4.34, P<0.01).Conclusions:1,The levels of OPN were increased in the serum and peritoneal fluid from patients with endometriosis, it was correlated with r-AFS staging and increased with the extent of disease , but no relationship with menstrual cycle phase ,which probably plays some roles on the occurrence and development of EMS, particularly in the patients of superficial peritoneal endometriosis.2,The higher levels of OPN in the serum and PF from patients with EMS has a very important diagnostic value.