A Detection Of The β-lactamase, Aminoglycosides Modification Enzyme And Chlorhexidine-sulphanilamide Drug Resistance Gene In Klebsiella Pneumoniae

Objective:To investigate theβ-lactamase,aminoglycosides modification enzyme,chlorhexidine-sulphanilamide drug resistance gene,TMP drug resistance gene, integrase gene and Tn21/Tn501 transposon genetic mark in 20 strains of Klebsiella pneumoniae susceptible to imipenem. To investigate the affinity of 20 klebsiella pneumoniae(KPN) plants.Methods:29 genes were detected in 20 KPN plants. They were sixteen kinds of beta-lactamase genes, six kinds of aminoglycosides modification enzyme , chlorhexidine-sulphanilamide drug resistance gene and TMP drug resistance gene, three kinds of integrase gene and Tn21/Tn501 transposon genetic mark detection. And they were used as molecule marker for the sample clustering analysis and assay of the detected results.Results : Among 20 strains, the positive rates of blaTEM,blaSHV,blaCTX-M-1,blaCTX-M-9,blaOXA-1,blaDHA genes were 85%, 25%, 25%, 20%, 25% and 70%, respectively. One kind ofβ-lactamases genes was found at least in 19 strains of K. pneumoniae . Furthermore, there exist 1 strain simultaneously with 6 kinds ofβ-lactamases genes.Aminoglycosides modification enzyme genes was found at least in 19 strains of K. pneumoniae(95%).There are qacE△1-sul1 genes in 18 strains of K. pneumoniae(90%) ; TMP drug resistance gene,integrase gene and Tn21/Tn501 transposon genetic mark were all detectable. Clone transmission was shown through the sample clustering analysis.Conclusions The expression of many kinds of resistant genes is associated with the resistance toβ-lactams, aminoglycosides,quinolone antibacterials in the 20 strains of K. pneumoniae,which could be clone transmitted.