| Objective: To investigate the protective effect of mild hypothermia on pancreatic acinar cells stimulated with supramaximal caerulein and explore the mechanism.Methods:Pancreas fragments of rats were divided into four groups: normothermia-control group, hypothermia-control group, normothermia -caerulein group and hypothermia-caerulein group. Cells viability were measured in 1h,3h and 5h. The activities of amylase,lipase in supernatant,the activity of trypsin in homogenate,the intracellular Ca2+ concentration and lactate dehydrogenase(LDH)leakage were measured in 30min,60min,90min.Results:1,The activities of amylase and lipase increased gradually in NP group and were higher than those of NC group(P<0.01). In HP group, activities of the amylase and lipase increased gradually too, but lower compared with NP group in 30min,60min,90min(P<0.05,or 0.01).2,Compared with NC group, activities of trypsin were significantly higher in NP group(P<0.01). The activity of trypsin in 15min,30min were significantly lower in HP group versus NP group(P<0.05,or 0.01).3,The LDH leakages increased gradually in NP group and were higher than those of NC group in 60min and 90min(P<0.01). In HP group LDH leakages increased gradually too, but lower verse NP group in 60min,90min(P<0.05,or 0.01).4,The cells viability in NP group decreased gradually and were lower verse NC group(P<0.01). In HP group, cells viability were higher than those of NP group in 3h and 5h(P<0.05).5,The intracellular Ca2+ concentrations in NP group increased gradually and were higher verse NC group(P<0.01). In HP group, intracellular Ca2+ concentrations were lower than those of NP group in 30min,60min and 90min(P<0.05,or 0.01).Conclusion:1,Mild hypothermia can protect pancreatic acinar cells from caerulein induced injury.2,The mechanism of protection might correlate to the inhibition of trypsinogen activation3,Mitigation of calcium overload might be associated with the protective effect of hypothermia. |
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