The Empirical Study Of Endostatin In Multiplication Of Hepatoma Carcinoma Cell And Tumor Growth Of Nude Mice Bearing Cancer

Objective:The experiment studies some important pathogenesis of liver cancer by researching the inhibitory action of endostatin in proliferation of human hepatoma carcinoma cells SMMC-7721 and the tumor growth of nude mice bearing cancer.Besides,the test also studies the etiopathogenesis and influential factor of liver cancer in molecular level ,trying to find a new way of healing and controlling cancers and providing dependable theoretical foundation for clinical curing.Methods: Applying MTT cell proliferation assay to observe the depressant effect of endostatin in proliferation of human SMMC-7721 hepatoma carcinoma cells;building animal model by inoculating subcutaneously human SMMC-7721 hepatoma carcinoma cells and detecting the tumor growth of nude mice bearing cancer in different densities of endostatin;Applying RT-PCR to detect the diversify of VEGFmRNA which is expressed by human SMMC-7721 hepatoma carcinoma cells and tumor tissue of nude mice bearing cancer.Results:In vivo,the tumor weight and the expression of VEGFmRNA are lower in endostain groups.The volume of tumor in endostain groups increases more slowly.There is a significant deviation between endostain groups and control group(P<0.05);in vitro, there is no significant deviation of inhibition ratio and expression of VEGFmRNA of human SMMC-7721 hepatoma carcinoma cells,contrasting to control group(P>0.05).Conclusions:Endostatin has a significant depressant effect on the tumorous growth of nude mice which are planted with human hepatoma carcinoma cells;in vivo, endostatin has an invariably influence in the expression of VEGFmRNA;in vitro,there is no depressant effect on tumor cell multiplication.