Preparation And The Effects To Parameters Of Hemorheology And Vascular Intimal Hyperplasia After Vascular Bypass Surgery On Canine Of Rhubarb Injection

OBJECTIVE In this experiment,the effect on vascular intimal hyperplasia(VIH)of rhubarb injection was investigated after vascular bypass surgery correlating with hemorheology for mechanism of inhibitory action to VIH of rhubarb injection.METHOD The rhubarb injection was prepared by reflux extraction incorporating with petroleum ether extraction method and precipitation method,and was tested about active components and biological safety.Twenty native dogs were divided to four groups randomly:high-dosage group,low-dosage group,middle dosage group and control group.Models of autogenous vein-carotid artery 45°bypass was set up by microscopic surgery.During two weeks,administration of rhubarb injection was intravenous infusion from brachiocephalic vein everyday.Blood samples were collected at different time points after administration of rhubarb to detect hemorheology parameters,such as whole blood viscosity,plasma viscosity,blood reduced viscosity,erythrocyte aggregation index,erythrocyte rigidity index, erythrocyte deformation index,erythrocyte electrophoresis index,et.al.And then, incorporating with hemorheology parameters,the effect of rhubarb injection on VIH was analyzed with biomechanics and pathophysiology.RESULTS The rhubarb injection prepared in the lab was examined that total anthraquinones content was 8.7386mg·mL-1(emodic acid 0.2236 mg·mL^-1,emodin 0.8342 mg·mL^-1,aloe-emodin 0.4913 mg·mL^-1,chrysophanic acid 5.4013 mg·mL^-1),and tannin content no more than 0.005mg·ml^-1.There were no heat source in this injection and which passed the stimulation test successful.As the animal experiment test showed that low-dosage rhubarb injection could decreased the whole blood viscosity(WBV) remarkably(10s^-1,P＜0.05),and three weeks later it could reduced the whole blood reductive viscosity(WBRV) extremely remarkable(10s^-1, P＜0.01).Middle-dosage rhubarb injection could decrease whole blood viscosity (WBV),whole blood reductive viscosity(WBRV)(10S^-1,60S^-1,150S^-1),Erythrocyte Aggregation Index(EAI),Erythrocyte Rigidity Index(ERI),Erythrocyte Deformation Index(EDI).High-dosage could reduced whole blood viscosity(WBV)(10S^-1,60S^-1, 150S^-1),but there was no effect with whole blood reductive viscosity(WBRV), erythrocyte aggregation index(EAI),Erythrocyte Rigidity Index(ERI),Erythrocyte Deformation Index(EDI)(P＞0.05).There was remarkable reduce of intimal thickness of flow stagnation point between control group and groups of rhubarb injection.CONCLUSION:Rhubarb injection could decrease the whole blood viscosity,the whole blood reductive viscosity,Erythrocyte Aggregation Index,Erythrocyte Rigidity Index and Erythrocyte Deformation Index aider vascular bypass surgery. Hemorheology change was the physiological basis of that inhibitory action to vascular intimal hyperplasia.