Effects Of Ultrasound Combined With Cisplatin And Paclitaxel On Metastasis Of Ovarian Cancer Cells

BACKGROUD AND OBJECTIVESOvarian cancer is the leading cause of death in gynecological cancer. Chemotherapy plays an important role in treatment for ovarian cancer. The combination of cisplatin and paclitaxel is the first-line regime. Ultrasound has been adopted extensively in medicine. In recent years, investigations have manifested that ultrasound enhance the cytotoxicity of some anticancer drugs. The aim of this study was to explore effects of ultrasound combined with anticancer agents on metastasis.METHODSHuman ovarian cancer cell lines HO8910 and HO8910PM were used and following experiments performed:1. To determine the critical ultrasonic doses for HO8910 cells and HO8910PM cells. Colony formation and ultrastructure changes were investigated.2. To study cells survival after exposure to cisplatin and paclitaxel with serial concentrations, determining a critical content. The experiment was performed in eight groups: (1) group control; (2)group US, cells were insonated; (2)group DDP, cells were subjected to DDP; (3)group DDP +US, cells were exposed to ultrasound after adding DDP; (4)group Taxol, cells were exposed to Taxol; (5)group Taxol+US, cells were exposed to ultrasound after adding Taxol; (6)group DDP+Taxol, cells were treated by the combination of DDP and Taxol; (7)group DDP+Taxol+US, cells were treated as that in group DDP+ Taxol, and ultrasound was also employed. The cells killing rates were used to determine the cell number for migration assay.3. To investigates cells transfering ability in vitro.RESULTS(1) There was no obvious critical ultrasound lethal dose, we chose one whose killing rate was only 10% for both cells (HO8910 cell was sonicated for 10 seconds and HO8910PM for 5 seconds). No significant difference was observed between experimental groups and controlled groups in neither colony assay nor growth curves.(2) The tumor cells were sensitive to both drugs when they were treated respectively; increased drug concentrations led to decreased cells survival rates. The efficacies for both drugs were identical. Cells killing rate was at 10% when we adopted 0.5μg/ml DDP and 6μg/ml Taxol which were used in the following researches.(3) In groups DDP, Taxol and DDP+Taxol, US could not only potentiate inhibition on adhesion ability of HO8910 cells greatly (P<0.05) but also mobility and invasion of HO8910 cells to some extent. If other treatment factors were not changed, adhesion ability of HO8910 cells receiving US sonication was weaker than those without US (P<0.05).No cells penetrating during mobility and invasion experiments; combined effects of DDP and Taxol on cells killing and transfering were significant.CONCLUSIONUltrasound-induced cell killing did not result from temperature rise in but caviation; US could enhance cells killing of the DDP+Taxol group, while no cells transfering possibility. Combined effects of DDP and Taxol on ovarian cancer cells killing was significant and transfer inhibition was greatly enhanced by US in vitro.