| Depression is one of the diseases characterized by constant down in spirits,and it has been the globally usual mental disease.In the light of the report from the World Health Organization:Today,depression has become the fourth disease in the world,it will be more serious causing disability than ischemic heart disease when it comes to 2020.In America,the expense for depression has reached over 4 billion dollars per year.It is based on literature studies,that the stagnation of Gan– energy is the commonest diagnostic type of dispersion,and the abnormal dispersion of Gan is a basic pathogenesis of dispersion."Si-guan"points will be used in acupuncture experimental study. The effects of Electroacupuncture (EA) of"Si-guan"points on Gan in charge of dispersion in the depression model rats will be researched from hemorrheology,liver tissue, gastrointestinal function,memory function and sexual function . The function reason of curing dispersion of electroacupuncture is the aim of our study.Fourty male Sprague-dawley rats were randomly divided into there groups of 4 each: normal group,model group,medicine group and EA group.All rats except the control group singly housed and exposed chronically 21 days to a variety of mild unpredictable stressors, the rats of EA group accepted EA treatment at the same time,and the rats of medicine group accepted fluoxetine treatment at the same time.The results show that:1. Hemorrheology:The whole blood viscosity,plasma,the idex of crythrocytic aggregation and deformation,hematocrit,and plasma fibrinog- en of despression rats were increased significantly compared to the normal group (P<0.05);The hemorrheological quotas of EA and medication groups reduced significantly compared to the model group (P<0.05);There were not significant difference between the EA group and medication group(P>0.05). Electroacupuncture (EA) of"Si-guan"points can improve the hemorrheological characteristics of depression rats.2. Liver tissue:The expression of Bcl-2 protein of liver tissue in model group was obviously shorter,and the expression of p53 protein of liver tissue in model group was obviously stronger compared to the normal group (P<0.05).It gave that depression restrained the expression of Bcl-2 protein,strengthened the expression of p53 protein。guided the liver apoptosis . The positive expression of Bcl-2 protein of liver tissue in EA and medication groups increased significantly compared to model group (P<0.05);But the positive expression of p53 protein of liver tissue in EA and medication groups reduced significantly compared to the model group (P<0.05);There were not significant difference between the EA group and the medication group (P>0.05). It gave that Electroacupuncture (EA) of"Si-guan"points could strengthened the expression of Bcl-2 protein,restrained the expression of p53 protein,and restrained the liver apoptosis It was a important reason of protecting the liver.3. Gastrointestinal function:The levels of iNOS and NO increased significantly and the activity of GSH-Px reduced significantly in colon of the model rats compared to the normal group (P<0.05);also the levels of iNOS and NO reduced significantly,the activity of GSH-Px increased significantly in colon of EA and medication groups compared to the model group (P<0.05);There were not significant difference between EA group and medication group(P>0.05). Electroacupuncture (EA) of"Si-guan"points had protected the colon tissue function of the depression rats.4. Memory function:The activity of AchE increased significantly and the levels of NOS and NO reduced significantly in the brain tissue of the model rats compared to the normal group (P<0.05);the activity of AchE reduced significantly,the levels of NOS and NO increased significantly in the brain tissue of EA and medication groups compared to the model group (P<0.05);There were not significant difference between the EA group and the medication group(P>0.05). Electroacupuncture (EA) of"Si-guan"points had improved the brain memory function of rats with depression.5. Sexual function:All items (smelling and lapping,crawling backs) of the model rats were reduced significantly compared to the normal group (P<0.05); But all items (smelling and lapping,crawling backs)of EA and medication groups increased significantly compared to the model group (P<0.05); There were not significant difference between the EA group and the medication group(P>0.05). Electroacupuncture (EA) of"Si-guan"points had adjusded sexual function of the depression rats.It is considered based on analysis of materials obtained from experiment studies,that the abnormal dispersion of Gan is related to the abnormal regulation of hypothalamus-pitutary-adrenal gland axis. Concretely,related with the changes of multiple neurotransmitters and their compound enzyme,such as neuropeptides,hormones and cyclic nucleotide system,showing the characteristics of multiple links,multiple levels and multiple targets. The regulator reason of Electroacupuncture (EA) will be gone a step further study.
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