| Objective: The irritable bowel syndrome (IBS) were one kind of intestinal irritable disease because of intestinal hypersensitivity, refer to a group of the functionality intestines disorder syndrome including the abdominal pain, the distension of the abdomen, the diarrhea, which of causes and the mechanism is not yet expounded until now. Speeds up unceasingly along with the rhythm of life with the social competition intensely, the incidence of IBS of world is rising day by day, which has interfered with people's normal work, the study and the life seriously.The IBS cause of disease and the pathogenesis not yet expounded until now. At present we thought that the enhancement of internal organs sensitivity is one of IBS most important pathology physiology characteristics, which is he foundation and symptom diversification reason which the IBS patient symptom produces. In recent years many studies tell us that the mast cell play a critical role during the pathology and physiological process of intestinal irritable disease. After being activated, the mast cell might release many biological activity different mediums, which release may further enlarge each physiology and pathological reaction, which thus can caused various symptoms. Therefore, suppressing activation of the intestinal mast cell, causing its reduced medium, including histamine, P material quantities, and so on, is the key to treats the intestinal irritable disease.The pathogenesis of IBS is complex, involving many aspects, including the spirit, the diet, individual quality, and so on. The sole medicine clinical function is usually limit, which cannot satisfy the clinical request. The Chinese Traditional Medicine also believed that, the origin reasons the abdominal pain and diarrhea are complex, while the laws of treating are also many. Huazhuojiedufang(HZJDF) is a classics Chinese Traditional Medical prescription by famous Chinese Traditional Medical expert Professor Li Diangui, which summarizes after many year clinical practices, is composed of pulsatilla root, goldthread root, scutellaria root, ginseng, astragalus root, white peony root, bighead, tangerine peel, ledebouriella root, ash bark, flavescent sophora root, aucklandia root, coix seed, chinese angelica root, liquorice, etc, has the may effections, including removing the poisonous quality of any substance, relieving the depressed liver and invigorating the spleen, reducing fever and remove dampness. Through the long-term clinical observation, it has been proved to have good curative effect on IBS. In order to further probe into the pharmacological action mechanism of HZJDF in treating IBS, we observe IBS rat model behavior, the electricity physiology target, and the blood biochemistry, attempts discussing the effect and action mechanism of HZJDF on IBS, as well as the clinical practical application curative effect research, to provide scientific basis for giving play to superiority of multi-ways, the multi-targets and the whole treatment of Chinese traditional Medicine, and to exploit and utilize scitificly Chinese traditional Medicine, simultaneously to build the foundation for the new medicine development. MethodsTests one: To apply the acetic acid chronic stimulation in colon to manufacture visceral hypersensitivity Rat Model of IBS. The rats were divided into three groups: the childhood rat acetic acid chronic stimulation group (the NA group), the grown-up rat colon acetic acid chronic stimulation group (the AA group) and the blank control group (the NS group). After modeiiing, the visceral sensitivity measurement were carried on separately to each group of IBS rat model, which conclude the capability limens of the sacculus that caused abdomen-uplifting and back-arching and the time of contract of abdomen muscle in rats, to confirm the succeeds of model.Tests two: To discuss the effect and Mechanism of HZJDF on visceral hypersensitivity rat model of IBS. After have made the rat model successful, the rats were divided into the normal control group (A group), the model control group (B group), the dicetel control group (Group C), the low dosage of HZJDF group (Group D), the high dosage of HZJDF group (Group E). Different groups were received oral administration of different treatment for one month. The capability limens of the sacculus that caused abdomen-uplifting and back-arching and the time of contract of abdomen muscle in rats were observed. The levels of 5-hydroxytryptamine (5-HT), substance P (SP), and calcitonin gene related peptide (CGRP) were evaluated. ResultsTests one: In the 6th week of modeling, The capability limens of the AWR that cause abdomen-uplifting and back-arching of NS group are (0.70±0.10)ml and (0.94±0.16)ml, The capability limens of the AWR that cause abdomen-uplifting and back-arching of AA group are (0.76±0.06)ml and (1.02±0.88)ml, There is no difference between them (P>0.05). Those of NA group are respectively (0.43±0.06)ml and (0.61±0.08)ml, which is significant lower than other two groups (P<0.01); When being tested in capacity of 0.5ml, the phenomenon of contract of abdomen muscle in rats of NS group and the AA group is not obvious, but the times of contract of abdomen muscle in rats of the NA group rat reached (6.63±1.06) time /5min, There is significantly different among them (P<0.01). When being tested in capacity of 0.8ml, the times of contract of abdomen muscle in rats of the NA group were more than other two groups obviously (P<0.05). When being tested in capacity of 0.8ml, the times of contract of abdomen muscle in rats of the NA group were more than NS group obviously (P<0.05), while there is no difference does not have the significance between the NA group and the AA group (P>0.05).Tests two: In the aspect of behavior test, there is significantly different between the B group and A group in the capability limens of the AWR that cause abdomen-uplifting and back-arching after the medication (P<0.01, P<0.05), and there is no difference between the A group and other three groups (P>0.05); there is no remarkable difference among D group, E group and C group (P>0.05); The result of abdominal myo-electricity showed that, when being tested in capacity of 1.0ml and 1.5ml, the times of contract of abdomen muscle in rats of the B group were more than A groups obviously (P<0.01) after the medication. While there is no difference among D group, E group and C group and A group (P>0.05); D, E group compare with C group, not remarkable difference (P>0.05); Comparing with the model control group, every group have distinct improvement in behavior and the electricity physiology target; 5-HT and SP drops obviously in each treatment group; The content of CGRP increases than before.Conclusions⒈Visceral hypersensitivity Rat Model of IBS which was manufactured by the acetic acid chronic stimulation in colon is steady and practicable.⒉HZJDF could lower the level of 5-HT and SP, and increase CGRP of IBS model rats, especially high dosage of HZJDF.⒊The mechanism of HZJDF may be increasing the limen of pain of bowel, eliminating hypersusceptibility of bowel, and decreasing excitability of nerve cells by decreasing the contents of 5-HT and SP in IBS model rats.⒋HZJDF has the may effections, including not only removing the poisonous quality of any substance, relieving the depressed liver and invigorating the spleen, reducing fever and remove dampness, but also improving the function of immunity and microcirculation, eliminating the free radical, inhibiting the inflammatory reaction, and relieving pain. It has superiority of multi-ways, the multi-targets and the whole treatment of Chinese traditional Medicine to treat with IBS. |
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