MR Perfusion Imaging Evaluation Targeting EBV-LMP1 MRNA Deoxidation Ribozyme-assisted Radiotherapy Nasopharyngeal Carcinoma's Clinical Study

Obieetive:Using MR perfusion imaging to evaluate targeting EBV-LMP1 mRNA deoxy ribozyme radiotherapy effect of the NPC.Methods:All these sections,which including 31 patients were all originated from Xiangya Hospital,Central South University from March 2007 to May 2008,were pathological diagnosised Nasopharyngeal cancer.They were divided into groups by double-blind,which including 17 patients in the testing group,14 patients un the contrasting group.The comprehensive treatment group use the EBV-LMP1 mRNA deoxyribozymes targeted therapy and radical radiotherapy,the radiotherapy alone group use placebo radical radiotherapy.All these patients were checked by MRI and PWI before radiotherapy,radiotherapy 50Gy,radiotherapy 70Gy and after 3 months of radiotherapy ending.The curative effect of EBV-LMP1 mRNA deoxyribozymes targeted therapy was analysis by observing the regression of tumor and changing the dabbling parameter,which before radiotherapy,in the radiotherapy and after radiotherapy.PWI using Turbo FLASH imaging sequence.Mission elbow intravenous injection Gd-DTPA(0.4mmol/kg),and the injection rate is 4 ml/s.TIC will be carried out after processing,and getting perfusion parameters,such as ER,Catake,EP,TTP.In conventional transverse- enhanced MRI,Sketched tumor borders and getted the mass volume,calculation of mass ratio.Results:During the three times,between the comprehensive treatment group masses reduced rate(the average rank:15.33,14.92,16.5) and the radiotherapy alone group masses reduced rate(the average rank: 9.67,10.08,8.5)had significant differences in the mass reduced rate.(P＜0.05).(?)In radiotherapy 50 Gy,the comprehensive treatment group's E_R (4.01±2.29/min)and Catake(287.41±101.54ml/100cm~3)was significantly lower than the radiotherapy alone group's E_R(8.08±4.23/min),Catake(384.91±42.36 ml/100cm~3)(P＜0.05).(?)In radiotherapy 70 Gy,the comprehensive treatment group's E_R(2.70±1.48/min),Catake(209.35±63.81 ml/100cm~3)and EP(2.59±0.70%)was significantly lower than the radiotherapy alone group's E_R(6.06±3.21/min),Catake(338.99±108.75 ml/100cm~3)and EP(3.41±0.65%)(P＜0.05)(?)After 3 months of radiotherapy ending,the comprehensive treatment group's Catake(176.43±118.05 ml/100cm~3) was significantly lower than the radiotherapy alone group's Catake (347.09±153.15 ml/100cm~3)(P＜0.05).Conclusion:①Use PWI to get ER,Catake,EP and TTP,will help evaluation of tumor's blood vessels leakage,judge radiation therapy,the outcome and prognosis has important clinical value,PWI is expected to become a mass prognosis of clinical judgement,by the effect of Election indicators.②Use MRI to evaluate the tumor size,shape,border and metastasis,will help to clear the masses border,staging mass and guiding radiotherapy has important clinical value.③The comprehensive treatment than the radiotherapy alone group may be more conducive to Reduce tumor size and vascular lesions of the leakage.