Study On Parp-1 And P53 Gene Polymorphisms Associated With H.pylori Infection In High Incidence Area Of Gastric Cancer In Gansu Province

Objective 1.This study was designed to investigate the association between PARP-1,P53 genotype and susceptibility to gastric cancer in Han Chinese population in he'xi area of Gansu province;2.To investigate interaction of PARP-1,P53 genotype and Hp infection;3.To investigate the association between PARP-1,P53 genotype and Lauren's histological typing and locating in gastric cancer.Methods 1.The genetic polymorphisms of PARP-1 and p53 genes was detected by realtime-PCR in 140 patients with gastric cancer(GC),110 patients with gastric precancerous lesion(GPL)and 125 healthy controls;2.HP infection was detected by Warthin-Starry' method;3.Data was mainly analysised by X~2 test and Logistic regression analysis in statistics.Results 1.The Arg allele frequencies of PARP-1 gene and Pro allele frequencies of p53 gene among GC cases(Arg,0.12;Pro,0.54)were higher than those among GPL and controls;The Arg allele and Pro allele had a more than 2.3-fold(OR=2.354,95%CI 1.140-4.861,P=0.018)and 1.8-fold(OR=1.846,95% CI 1.006-3.387,P=-0.046)increased risk of gastric cancer,respectively;2.A significantly higher risk of gastric cancer was also seen in cases with Arg genotype and Pro genotype and HP infection,positive family history than in healthy controls(P＜0.05);3.With statistical analysis,the Arg genotype and Pro genotype frequencies was highly significant different in cardiac adenocarcinoma and Lauren's histological type(P＜0.05).Conclusion 1.PARP-1 Arg genotype or p53 Pro genotype contributes to the increased susceptibility to gastric cancer;2.PARP-1 Phe54Leu genotype is associated to elevated risk of Arg genotype or p53 Pro genotyp to gastric cancer,though itself is not correlation to gastric cancer;3.These results indicate that there are multiplicative joint effects between PARP-1 Lys940Arg and Hp infection,p53 Arg72Pro and Hp infection in the risk of developing gastric cancer;4.Gene-gene interaction of PARP-1 Lys940Arg,Lys940Arg and p53 Arg72Pro polymorphisms increased risk of gastric cancer in a supermultiplicative manner.