Detection Of Integron, Homology And Genotype Of Carbapenemases In Multi-drug Resistance Acinetobacter Baumannii Isolated From Burn Unit

Objective To investigate the relationship between antibiotic resistance, integron, homology and genotype of carbapenemases of Acinetobacter Baumannii collected from burn unit. Methods The minimum inhibitory concentrations (MIC) of these strains were examined by agar dilution. Homology of these strains were analyzed by pulse-field gel electrophoresis (PFGE), Class 1, 2 and 3 integrase, integron genes and genotype of carbapenemases were detected by PCR and verified by DNA sequencing. Results Thirty one strains of Acinetobacter Baumannii were highly resistant to all of the antibiotics except imipenem, meropenem, cefoperazone-sulbactam, piperacillin-tazobactam. They were classified into 3 clones based on PFGE pattern. Integrons harbored aadA1, aadA5, aacA4, aac3, catB8, aacC1, aac (6')-Ib, drfA17 and drf8 genes. Conclusions Multi-drug resistance Acinetobacter baumannii of clone A is spreading widely in burn unit of People's Hospital of Gansu Province. Integrons harbored the genes hydrolyzing aminoglycosides, rifampin, and chloram-phenicol. All of the carbapenem -resistant Acinetobacter Baumannii was producing OXA-23 carbapenemase.