Effects Of Monoclonal Antibody To Integrin αvβ3 On Cell Proliferation,Invasion And Apoptosis Of MDA-MB-435 Cells

Objectives:To investigate the effects of monoclonal antibody to integrinαvβ3 on cell proliferation and apoptosis of MDA-MB-435 cells .Methods:immunohistochemistry staining (SP)was used to examine the expression of integrinαvβ3 receptor in breast cancer cell lines MDA-MB-435s.Cell growth inhibition of monoclonal antibody to integrinαvβ3 on MDA-MB-435 cells was analyzed by MTT assay.Cell migration assays were performed in 24-transwell chambers with a polystyrene membrane (6.5-mm diameter, 10-μm thickness, and 8-μm pore size).Cell apoptosis was detected by Hoechst 33258 staining after monoclonal antibody to integrinαvβ3 treatment for 48 hours;apoptotic rate were analyzed by cytometry.Results:Integrinαvβ3 expression in cellular membrane was high in MDA-MB-435s cell lines.monoclonal antibody to integrinαvβ3 could inhibit the proliferation of MDA-MB-435 cells in a time-and dose-dependant manner. monoclonal antibody to integrinαvβ3 could inhibit cell invasion.Hoechst 33258 staining indicated that apoptosis was induced by monoclonal antibody to integrinαvβ3 After treated for 48 hours, And apoptosis rate increased in dose-dependant manner.Cell apoptosis rate of monoclonal antibody to integrinαvβ3 groups were significantly higher than that of control group(P<0.01).Conclusions:monoclonal antibody to integrinαvβ3 has not only targeted neovascularization but also direct tumor-killing effects. integrinαvβ3 antagonist is an ideal therapeutic agent for potential tumor targeting therapy.