Correlation Of Interleukin 4-590 And Intron 3 Polymorphisms To Genetic Susceptibilities To Gastric Cardiac Carcinoma And Noncardiac Gastric Carcinoma

Objective This study was to investigate the correlation of interleukin 4-590 and intron 3 gene polymorphisms to susceptibilities to gastric cardiac carcinoma and noncardiac gastric carcinoma in Fujian province.Methods Subjects were divided into three groups: All the subjects were recruited by pathological diagnosis and excluded from autoimmune disease,including 100 gastric cardiac carcinoma patients,130 noncardiac gastric carcinoma patients and 113 control subjects.Interleukin 4-590 and intron3 polymorphisms were genotyped by polymerase chain reaction(PCR) and Sanger sequencing.The results were analyzed by SPSS13.0 and EH software.Results 1.The number of the subjects with family history of upper gastrointestinal cancer(UGIC) was significantly higher in gastric cardiac carcinoma group and noncardiac gastric carcinoma group than in control group(P<0.05).Family history of UGIC may increase the risk of developing gastric cardiac carcinoma and noncardiac gastric carcinoma. 2.The overall genotype distributions of interleukin 4-590 in noncardiac gastric carcinoma group were significantly different from those in control group(P<0.01).However,there was no significant difference between gastric cardiac carcinoma group and control group(P>0.05). Compared with individuals with C/C genotype,individuals with T/T genotype had significantly higher risk in developing gastric cardiac carcinoma, but not in developing noncardiac gastric carcinoma;individuals with C/T genotype couldnot change risks of developing both gastric cardiac carcinoma and noncardiac gastric carcinoma. When stratified for gender and family history of UGIC,compared with individuals with C/C genotype,individuals with T/T genotype in male group and in the group without family history of UGIC had higher risk in developing gastric cardiac carcinoma. 3.The overall genotype distributions of interleukin 4 intron 3 in gastric cardiac carcinoma group and noncardiac gastric carcinoma group were significantly different from those in control group(P<0.01and P<0.05,respectively). Compared with individuals with RP1.1 genotype,individuals with RP1.2 genotype had significantly higher risk in developing noncardiac gastric carcinoma, but not in developing gastric cardiac carcinoma.When stratified for gender and family history of UGIC,compared with individuals with RP1.1 genotype, individuals with RP1.2 genotype in male group and in the group without family history of UGIC had higher risk in developing noncardiac gastric carcinoma ,individuals with RP2.2 genotype in the group without family history of UGIC had lower risk in developing noncardiac gastric carcinoma. 4. There was linkage disequilibrium between -590 and intron 3 in interleukin 4 gene.The haplotype distribution of gastric cardiac carcinoma patients was not significantly different from that of controls(P>0.05),while the haplotype distribution of noncardiac gastric carcinoma patients was significantly different from that of controls(P<0.01). Compared with C/RP1 haplotype,T/RP1 and T/RP2 haplotypes significantly decreased the risk of developing noncardiac gastric carcinoma.Conclusions 1. Family history of UGIC might increase the risk of developing gastric cardiac carcinoma and noncardiac gastric carcinoma.2.Interleukin4-590 C/T polymorphisms were associated with the susceptibility to gastric cardiac carcinoma.3. Interleukin 4 intron 3 polymorphisms were associated with the risk of developing noncardiac gastric carcinoma. 4. There was linkage disequilibrium between -590 and intron 3 in interleukin 4 gene. T/RP1 and T/RP2 haplotypes might decrease the risk of developing noncardiac gastric carcinoma.