Significance And Expression Of Matrix Metalloproteinases9 Production In Cholangiocarcinoma Cell Nerval Metastasis Potential

Objective To study the Significance of matrix metalloproteinases activity in cholangiocarcinoma cell with different lymphatic and nerval metastasis potential.Methods Sandwich enzyme-linked immunosorbent assay(ELISA) was used to detect the difference of MMP-9 product and activities in cholangiocarcinoma cell line(QBC939) that were incubated in serum-free medium,homogenate of lymph node or nerve tissue,respectively.Results After incubation in the serum-free medium,the production of MMP-9 was at the low level in QBC939(19.93±2.94).AfterQBC939 were incubated in homogenate of lymph node,nerve the expression of MMP-9 was upregulated apparently,which were(27.33±3.98),(27.16±4.11) respectively,higher than that of the serum-free medium(P＜0.001).Conclusion:Hostmicroenvironment could contribute to the increasing of MMP-9 in QBC939,which is closely correlated to their specific lymphatic nerval metastasis potential.