| Objective To investigate the effect of Tripterygium wilfordii(TW) on nitric oxide(NO), inducible nitric oxide(iNOS) synthase and Total Antioxidative Capability in asthma rats.Methods Thirty-two Wistar male rats were randomly divided into control group, asthma group and TW treated group. The iNOS mRNA was measured by RT-PCR and the level of iNOS in airway wall was measured by immunohistochemistry. NO concentration and total antioxidative capability in serum and lung tissues of asthma rats were detected.Results The expression of iNOS mRNA and iNOS protain in asthma group was higher than in control group and TW treated group(P<0.05); the expression of iNOS mRNA and iNOS protain in TW treated group was higher than in control group(P<0.05); The NO concentration in serum and lung tissues of asthma group were significantly higher than in control group and TW treated group(P<0.05), but total antioxidative capability in serum was lower than in control group and TW treated group(P<0.05). The NO concentration in serum and lung tissues in TW treated group were higher than in control group(P<0.05), but total antioxidative capability in serum in TW treated group was lower than control group(P<0.05).Conclusion Tripterygium wilfordii can inhibite the expression of iNOS mRNA and may enhance antioxidative capability in asthma rats through reducing NO concentration. |
PDF Full Text Request