| Betelnut is a kind of traditional Chinese medicine, classified into medicinal and edible betelnut. Betelnut, cigarette and chutty have been considered as popular mouth hobby staple in many countries, and the demand become larger and larger. Based on high sensitive, specificity, credibility of liquid chromatography-mass spectrometry (LC-MS) and simple sample preparation, our work provide reliable qualitative and quantitative results for betelnut analysis. The main contents are as follows:1. Development status and current problems of betelnut food has been summarized. The advantages of HPLC/ESI-MS technique and its application in food detection have been discussed.2. Arecoline is the main function component of betelnut. We obtained optimum extraction conditions by research on prepared sample. Combining with computer index system and mass spectrometry theory, we qualitative analyze betelnut extract by LC-MS and mass spectrometric analyze arecoline. Furthermore we prepared arecoline by thin layer chromatography, and quantitative determined arecoline by LC-MS. The pretreatment was simpler.3. By spectrophotometry, volumetric determination and LC-MS, the quantitative analysis of arecoline was obtained. The quantitative result by LC-MS was higher than that by spectrophotometry and volumetric determination.4. Ephedrine and methylephedrine is the main constituent of ephedra. Ingesting some ephedra will do harm to health. So it is forbidden to add ephedra into foods. LC separation of ephedrine and methylephedrine was established, and it improved anti-interference ability and detection limit. Furthermore, good reliability of qualitative analysis was obtained by research on the best MS conditions. The method was used to accurate determine ephedrine, methylephedrine and its metabolites as illegal food additives in betelnut. |
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