CD105, TGFβ1, COX-2 Expression And Significance In Breast Cancer

Background and Objective Breast cancer is a threat to the lives of women as a common disease.In recent years due to improvement of living standards,lifestyle changes,the number of patients with breast cancer is gradually increasing.Some study find that the formation of new blood vessel is essential to the formation and development of breast cancer,therefore anti-angiogenesis treatment becomes a major emerging field.Now as the tumor marker for the reagent in microvascular CD105 is the most reliable.CD105 involves in blood vessel growth,plays an important role in the process of tumor blood vessel growth,and has relation to the transfer and prognosis of cancer.CD105 will play an important role in the diagnosis and treatment of cancer.TGFβ1 is a multifunctional cell growth factor,in the early stages of tumor,cells lost TGFβ1-mediated growth inhibition due to one or more of TGFβ1 signaling pathway loss of cytokines,in the late stage of tumor growth,as a factor in promoting tumor,TGFβ1 provide tumor with micro-environment for growth,invasion and the transfer by stimulating angiogene-sis,cell migration,immune suppression and synthetic extracellular matrix and so on.Another,COX-2 is one of tumor markers,COX-2 inhibits endothelial cells in apoptosis and promote angiogenesis through the suppression of arachidonic acid accumulation,Through detecting CD105,TGFβ1 and COX-2 protein in breast normal tissue,dysplasia,invasive cancerⅠand invasive cancerⅡ-Ⅲtissues,this study explored them by the role and mutual relations in the course of breast cancer,in order to provide more theoretical basis for clinical treatment. Methods Anti-streptomycin-peroxidase linked Immuno histochemist- ry(S-P) method being adopted to detect CD105,TGFβ1,COX-2 protein expression in breast normal tissue group(25cases),breast dysplasia group (27cases),invasive breast cancerⅠ(29cases) and invasive breast cancerⅡ-Ⅲ(36 cases).Results 1.CD105 expression mainly targets angiogenesis in the endothelial cells and angiogenesis unevenly distributs,mostly confineds to the edge of the tumor in real terms within the interstitial.CD105 expression in breast cancer has no relativity to patient ages and tumor size. The MVD marking CD105 protein respectively were 6.50±1. 59,19.30±4. 26, 38 .16±5. 54, 57. 88±9. 47 in the normal group,dysplasia group, invasive cancerⅠgroup and invasive cancerⅡ-Ⅲgroup,more significant difference between them (P <0.05).2. TGFβ1 expression mainly locateds in the cytoplasm of cancer cells and cancer cells in the edge of the tumor strongly express, the certain vascular endothelial cells also have positive expression.TGFβ1 expression in breast cancer has no relativity to patient ages and tumor size. TGFβ1 protein expressions in the normal group,dysplasia group, invasive cancerⅠgroup and invasive cancerⅡ-Ⅲgroup have expressed positive rates,respectively were 24.0%,44.5%,65.5% and 88.9%, more significant difference between them( P <0.05).3. COX-2 protein expression mainly locateds in the cytoplasm of cancer cells,endothelial cells,fibre cells and smooth muscle cells scatterly have positive expression.COX-2 expression in breast cancer has no relativity to patient ages and tumor size.The positive rates of COX-2 protein are close, respectively 48.0%,55.2%and55.6% in the normal group, invasive cancerⅠgroup and invasive cancerⅡ-Ⅲgroup,shows no significant difference between them(P>0.05), The positive rates of COX-2 protein in dysplasia Group is 85.2%, and is significantly different with the normal group, invasive cancerⅠ, invasive cancerⅡ-Ⅲgroup (P <0.05).4. In dysplasia group,the MVD marking CD105 protein in TGFβ1 positive expression group(21. 50±1. 98) is significantly higher than in TGFβ1 negative expression group(9. 88±3. 39)(P<0.05).In invasive cancerⅠthe MVD marking CD105 protein in TGFβ1 positive expression group (44. 80±7. 29) is significantly higher than in TGFβ1 negative expression group(25. 50±1. 33) (P <0.05).In invasive cancerⅡ-Ⅲthe MVD marking CD105 protein in TGFβ1 positive expression group (65. 23±5. 75) is significantly higher than in TGFβ1 negative expression group (32. 10±7. 94) (P <0.05).5. In dysplasia group,the MVD marking CD105 protein in COX-2 positive expression group (26. 70±5. 38) was significantly higher than in COX-2 negative expression group (13. 80±3. 55) ( P <0.05). In invasive cancerⅠthe MVD marking CD105 protein in COX-2 positive expression group (46. 25±4. 67) was significantly higher than in COX-2 negative expression group (27. 57±2. 63) (P < 0.05).In invasive cancerⅡ-Ⅲthe MVD marking CD105 protein in COX-2 positive expression group (68. 93±3. 95)was significantly higher than in COX-2 negative expression group (43. 68±5. 84) (P <0.05).6. In dysplasia group TGFβ1 protein expression has no significant correlation with COX-2 protein expression (P> 0.05).In invasive cancerⅠTGFβ1 protein expression has no significant correlation with COX-2 protein expression (P> 0.05).In invasive cancerⅡ-ⅢTGFβ1 protein expression has no significant correlation with COX-2 protein expression (P> 0.05).Conclusion 1. The MVD marking CD105 protein gradually increase in the normal group,dysplasia group, invasive cancerⅠgroup and invasive cancerⅡ-Ⅲgroup. It suggests that the MVD marking CD105 protein be related to the degree of tumor differentiation, the more differentiation Poor, more higher CD105 expression, more stronger inducing angiogenesis ability.It also suggests angiogenesis plays a role in breat cancer.There is a huge significance for the early clinical diagnosis and estimating prognosis.2. The positive rate of TGFβ1 protein gradually increase in the normal group,dysplasia group, invasive cancerⅠgroup and invasive cancerⅡ-Ⅲgroup. It suggests that TGFβ1 is likely to improve breast cancer developmemt. It also suggests TGFβ1 plays a role in breat cancer.There is a huge significance for the early clinical diagnosis and estimating prognosis3. In dysplasia group, The positive rate of COX-2 protein is significantly higher than in the normal group, invasive cancerⅠgroup and invasive cancer Ⅱ-Ⅲgroup and within the three groups there are no significant difference.It suggests that COX-2 protein expression may be breast cancer early and testing COX-2 may contribute to the early clinical diagnosis.4. TGFβ1 protein and COX-2 protein in breast cancer development may play in a role both through inducing angiogenesis.But the result suggests that these two proteins may be relatively indep endent of the incident. In the development of different periods in breast cancer, they may be induced by various means to play a role in angiogenesis.