The Effect Of Placenta-derived Mesenchymal Stem Cells On T Lymphocytes Proliferation And Cytokines Secretion In Vitro

Objective: The mesenchymal stem cells(MSCs) have self-renewal and multi-directional differentiation characteristics , which will be widely used in the transplantation, gene therapy and the tissue engineering. But the source and the immuno-regulatory mechanism of MSCs need to be resolved. In this study, we isolated and cultivated mesenchymal stem cells from human placenta tissue, and then investigate of the effect of MSCs from human placenta tissue on allogeneic T lymphocyte proliferation and cytokine secretion.Method: Firstly we isolated human placenta-derived mesenchymal stem cells(PMSCs) from placenta tissue, observed their morphology and identified cell surface antigen expression by FACS analysis and their differentiation potency in vitro. The PMSCs were cultivated and expanded in vitro, then added to two-way mixed lymphocyte reation(MLR) essay, according to different ratio of PMSCs to lymphocytes. The secretion of IL-2,IL-4 and IFN-γin each group are evaluated by ELISA after 3 days'coculture, and the T lymphocyte proliferation is measured after 6 days'coculture. Result: MSCs isolated from placenta tissue had the similar morphology and cell surface markers with bone marrow mesenchymal stem cells(BMSCs). CD29, CD44 and CD105 expressions were found on PMSCs but not CD34,CD45,CD106 and HLA-DR. PMSCs also can be induced into neuron-like cells. In MLR essay, PMSCs can be inhibited allogeneic T lymphocytes proliferation in vitro and affected IL-2, IL-4 and IFN-γsecretion.Conclusion: The human placenta tissue is proved to be a useful source of the MSCs. PMSCs had the similar biological character and immuno-regulatory effect with BMSC, which will provide alternative cell source for the mesenchymal stem cells.