Study Of Dynamic Pathological Change In The Early Period Of Liver Injury Caused By Choletasis In The Rats

【Objective】To observe the early dynamic pathological change in bile duct epithelial cells in obstructive jaundice and to determine the time point of liver fibrosis,and also to discuss the role of the bile duct epithelial cells in the development of liver fibrosis by the immunohistochemistry study of CK-19,TGF-β₁,typeâ…£collagen.【Methods】We ligated the common bile duct in the rats to establish the liver injury model.Rats were divided into 5 groups:â… (control group),â…¡(lst day after the ligation),â…¢(3st day after the ligation),â…£(7st day after the ligation),â…¤(14st day after the ligation).Samples were collected from the different groups.The levels of serum(Tbil,ALP,GGT,Alb)were tested,and HE stainning and Immunohistochemistry stainning of CK-19,TGF-β₁,typeâ…£collagen were performed in the rat's liver tissues.【Results】①Serum Alb decreased compared with sham-operated group;GGT and TBil increased continuously after ligation of the common bile duct,ALP increased first and dropped a little bit after one week.â‘¡Histopathology in liver:Epithelial cells of Intrahepatic bile duct became flat after ligation and more epithelial cells appeared around acini hepatis after 3 days,the new bile ducts formed after 1 week,hepatic fibrosis was starting around acini hepatis after 2 weeks,but fiber intermission was not completely formed.â‘¢Immunohistochemistry study of CK-19,TGF-β₁ typeâ…£collagen in hepatic tissue:CK-19 in epithelial cells of small bile duct surrounding the Ministry of lobular showed weak positive after 3 days of ligation.The number of bile duct epithelial cells increased and CK-19 expression became stronger in one week. New small bile appeared after two weeks,in the mean time CK-19 expression became even more stronger.Base membrane of bile duct and collagen layer were thickened after one week.Small fiber compartment extended but fiber interval was not completely formed after 2 weeks.TGF-β₁ expression increased evenly in liver tissue after 1 week and reached the strongest expression after 2 weeks.【Conclusion】①The new epithelial cells appeared after 3 days ligation of the common bile duct,and new bile duct formed later.â‘¡The formation of the new bile duct was bile duct,and new bile duct formed later.â‘¡The formation of the new bile duct was accorded with TGF-β₁ expression.And epithelialis cells of bile ducts were one of the chief activated cells in the development of hepatic fibrosisâ‘¢Quasi- hepatic fibrosis was formed after 1 week ligation of the common bile duct.After 2 weeks small fiber compartment extended but fiber interval was not completely formed yet.That may mark the beginning of the hepatic fibrosis.