Detection And Correlation Analysis Of Lipopolysaccharide, Vitamin D Receptor And Matrix Metalloproteinase-9 In The Middle Ear Cholesteatoma

Objective: To investigate the expression and concentration of lipopolysaccharide, vitamin D receptor and matrix metalloproteinase-9 in middle ear cholesteatoma and discuse their relations.Methods: 29 cases of middle ear cholesteatoma tissue we studied were come from the patients who were operated in First Affliliated Hospital of Guangxi Medical University, 18 cases of external auditory canal tissue were acquired from those patients at the same time, and all of them had been verified by pathology. Tissues were stored into 2 parts, ones were stored without any additive in pyrogen-free tubes at -80 C until further processing, the other ones were formalin-fixed, paraffin-embedded for immunohistochemical test. The concentrations of LPS were detected by limulus amebocyte lysate assay (LAL-assay) , and expression of VDR and MMP-9 protein in formalin-fixed, paraffin-embedded tissues were detected by immunohistochemical method.Results: The result showed that the concentrations of LPS in cholesteatoma are higher than in external auditory canal tissues (the numbers are logarithmed): Group of Cholesteatoma: M=0. 7390, IQR=0. 6203; Group of external auditory canal tissues : M=-0.2538 , IQR=1. 1692 (P<0.05) . Between the two-type groups: Group of intensive type: M=0. 8403, IQR=0. 5254; Group of localized type: M=0. 4048, IQR=0. 6139, the concentrations of LPS were higher in extensive cholesteatoma in comparison with localized cholesteatoma (P<0.05) . VDR was located at karyon, taking on yellow brown pellet, could be detected in all external auditory canal epithelium but there were less positive cells in middle ear cholesteatoma. In cholesteatoma epithelium samples, VDR were 93.1%. MMP-9 was located at cytoplasmic, taking on yellow brown pellet, could be detected in almost middle ear cholesteatoma epithelium but there were less positive cells in external auditory canal epithelium. In cholesteatoma epithelium samples, MMP-9 were 79.3%. Compared with external auditory canal epithelium, the expression of MMP-9 was higher in middle ear cholesteatoma epithelium (P<0.05), but was lower in expression of VDR(P<0.05). They didn't have significant difference in two types of cholesteatoma epithelium(P>0.05). LPS, MMP-9 and VDR weren't significant correlated by Spearman test.Conclusion:1 The concentrations of LPS were higher in cholesteatoma compared with external auditory canal, and were higher in extensive types compared with localized ones, which hints LPS was responsible for middle ear cholesteatoma and the bone erosion of cholesteatoma.2 The expression of VDR was higher in external auditory canal compared with cholesteatoma, which hints VDR was responsible for middle ear cholesteatoma.3 The expression of MMP-9 was higher in cholesteatoma compared with external auditory canal, which hints MMP-9 was responsible for middle ear cholesteatoma.4 There's no correlation among LPS, VDR and MMP-9, which hints the relationships among the three were unkown.