The Study Of The Oxidation Stress Effect Of Ganzhikang On Non-alcoholic Fatty Liver

Objective: To study the mechanization of the action of Gangzhikang on non-alcoholic fatty liver through the systematic clinical and animal experiment by observation of the change of TC,TG,ALT,MDA,SOD of both the patients and the animals after taking Gangzhikang, and the change of the CPY II E1 and histopathology indicator of the animal liver.Methods: In the clinical experiment the traditional Chinese medicine group and the control group were set and the randomized block design and x2-test were applied. The observation group (with 74 patients) was treated with the compound herb medicine, Ganzhikang, which can nourish the liver and reduce the blood-fat. Taking one month as a treatment course, after 2 to 3 courses of treatment, it was counterchecked that the multi-colored ultrasonic of B-type and blood biochemistry of the patients. The control group (with 47 patients) conventionally took Dongbao Gankang, 3 tables per time, 3 times per day, with the same treatment course.In the animal experimental study 5 groups were divided: the high dosage Ganzhikang gruop, the low dosage Ganzhikang group, the Dongbao Gankang control group, the model control group and the blank control group. The observation contained ALT, TC, TG, MDA, SOD, hepatic histopathology indicators and so on.Results: In the clinical group, the total effective percentage of the treatment group is 91.89%, while it was 74.46% in the control group thus the healing efficacy of the treatment group was markedly superior to that of the control group.The outcome of the animal experiment: The hepatic histopathology result showed that the curative effect of the Ganzhikang low dosage group was better than that of the Ganzhikang high dosage group while the therapeutic effectiveness of the traditional Chinese medicine treatment groups was all superior to that of the Dongbao Gankang control group. The blood-fat and ALT of the rats in the model group increased markedly compared with the blank control group (P<0.01); the MDA of the rats in the model group increased markedly in compare with the blank control group and the Ganzhikang group (P<0.01); the SOD of the rats in the model group decreased notablely compared with the blank control group and the Ganzhikang group (P<0.01); the CYP 2E1 of the hepatic tissue and its gene expression of the rats of the model control group increased markedly in compare with the blank control group (P<0.01); the effect on MDA,SOD and CYP2E1 of Ganzhikang treatment group and Dongbao Gankang control group were similar and without statistical significance.Conclusion: The oxidation stress effect of the Ganzhikang treatment group on non-alcoholic fatty liver is similar with the Dongbao Gankang control group's and without statistical significance. ALT and histopathology is better than that of the Dongbao Gankang control group and the model control group. The clinical study has pointed out that Ganzhikang has satisfying and exact curative effect on non-alcoholic fatty liver. It can be inferred that Ganzhikang can decrease TC, TG, resist lipid peroxidation and promote the recovery of the impaired hepatic tissue, so as to cure the non-alcoholic fatty liver.