Research On High Expression Of Type I Collagen In Gastric Cancer And Esophageal Cancer

Gastric cancer (GC) is one of the most common digestive tract cancers in China, and its mortality rate is on the top of malignant tumor with 25.21/100000. Therefore, studies on molecular mechanism and therapy of GC have been on the hot spot of medical reaserch. Up to now, some of GC relative genes have been not only widely studied but also applicated in judgement of diagnosis, therapy and prognosis of GC. However, other brand new GC relatived genes are waited to be deeper understood at more aspacts. In order to approach to those specific expressed genes during GC development, fluorescence differential display(FDD) is utilized to screen out the genes that are expressed differently between gastric cancer tissue and the corresponding normal mucosa tissue in our study, also, Semi-quantitative RT-PCR,fluorescence quantitative PCR are used to define these genes. Now, we have got several results as follows:1. With the help of FDD-PCR, we got three different bands based on 20 cases of gastric cancer tissue and the corresponding normal tissue, and finally we had two steady different bands using PCR for further amplification.2. After sequencing these two bands, we cloned the two cDNA labled as W1 (226bp),W2 (175bp) respectively. Then the two sequences were analysied in NCBI BLAST database, and the result showed that W1 has 100% homology with Homo sapiens Type I collagen gene, W2 has 100%homology with Homo sapiens ribosomol protein L15 gene.3. fluorescence quantitative PCR was applied to check Type I collagen gene in different tissues, and GC has higher level of expression of Type I collagen gene than its the corresponding normal mucosa tissue.Furthermore,we repeated this assay in esophageal cancer and the corresponding normal mucosa tissue, and similar result was appeared in this cancer. More over, the Type I collagen gene expressed at a higher level in esophageal cancer than GC.4. Based on the same technology-- fluorescence quantitative PCR, five different cancer cells were tested, and Type I collagen gene was expressed almost the same in these five tumor cells. The data indicate that it maybe have universal expression in most tumors.