The Protective And Therapeutic Effects Of Compound Rhizoma Coptidis Capsule On Early Diabetic Nephyopathy Rats

OBJECTIVE:To research the preventive and therapeutic effect of compound Rhizoma coptidis capsule on early diabetic nephropathy rats.METHODS:1.Preparation and determination of CRCC:This experiment was designed on the basis of the preliminary work.We use established methods for preparation and determination of berberine hydrochloride and puerarin in compound Rhizoma Coptidis capsule。2.Establishment,grouping and administration of DN rats:The early diabetic nephropathy rats were established by intraperitoneal injection in these rats with streptozotocin in a dose of 60mg/kg.Rats which FBG≥11.1mmol/L are used and divided into six groups randomly.CRCC(2.18,4.36g/kg),berberine hydrochloride 200mg/kg combinating puerarin 150mg/kg,XKW(0.8g/kg),Enalapril(10mg/kg) was administr -ated via gavage daily throughout the experiment.3.Index detection:FBG,BUN,Scr are detected by biochemical analysis.The changes of Ins,C-P,TNF-α,α1-MG,β2-MG are reflected by RIA analysis.Light microscopy observe the glomerular HE staining and electron microscopy overview kidney ultrastructure.Immunohistochemistry express the renal cortex Bcl-2,Bax,VEGF protein content.RT-PCR test the expression of renal cortex Bcl-2,Bax,VEGF mRNA.RESULTS:The results showed that serum levels of FBG,Scr,BUN,TNF-α,α1-MG,β2-MG markly increased,meanwhile the levels of bodyweight,INS and C-P markly decreased in model rats.The model rats' glomerular cells showed vacuolar changed, extracellular matrix increased and tubular swelled observed under light microscope. Glomerular basement membrane thickening and foot process integration were observed through the electron microscope.In conclusion,we successfully prepared the early diabetic nephropathy rat model.CRCC(2.18,4.36g/kg) not only obviously improve the glomerular cells showed vacuolar changed and reduce the accumulation of extracellular matrix,but also lessense the extent of glomerular basement membrane thickening.These results suggest that CRCC has positively preventive and therapeutic effect on DN rat.Conclusion:To sum up,the streptozotocin induced early diabetic nephropathy rats model is more likely to reproduce the key features of early diabetic nephropathy in human.CRCC has positively preventive and therapeutic effect on experimental DN model.It can not only amendment the renal function,step down the blood glucose,heighten the insulinbut asoihas the ability to elevate the expression of Bc1-2 proteinum and mRNA, inhibit the Bax,VEGF proteinum and mRNA expression and protect the ultrastructural mitochondrial and renal lesion.