Study On The Relationship Between The Abnormality Of Cervical Vertebral Stabilization And Bcl-2/bax Gene In Mice

With more and more people aging, Alzheimer's disease has already become a serious social problem at present. Scientists have explored the cause and mechanism of Alzheimer's disease on more points of views. There are two main risk factors: interior factors and environmental factors. Our experimental groups have detected that the abnormality of cervical vertebral stabilization can drive the murine aging progress obviously on early stage because of soft tissue injury around murine cervical vertebra. Ethology detection manifests that murine learning and memory ability decrease obviously;immunohistochemistry detection displays that amyloid-βexpress is positive and the senile plaques of brain tissue are more compared with those of control's; it shows that the abnormality of cervical vertebral stabilization was one of main risk factor of Alzheimer disease.Objective: In order to approach the molecular biology mechanisms between the abnormality of cervical vertebral stabilization and Alzheimer's disease. We observed the expressions of bcl-2,bax and caspase-3 in brain at mRNA level with RT-PCR and protein level with Immunohistochemistry and Western blot.Method: brain tissue cells'death is observed by HE staining; the protein expressions of bcl-2 , bax and caspase-3 are detected by Immunohistochemistry and Western blot;the mRNA expressions of bcl-2,bax and caspase-3 are Investigated though RT-PCR.Results :HE straining of brain tissue: Compared with the normal groups there are sporadic or consecutive nerve cells death obviously in the hippocamp of model group mice, showing kytoplasm anachromasis ,karyopycnosis,the fuzzy of cell membrane and nuclear membrane, even Punctiform interruption. Immunohistochemical staining of brain tissue: there are less bcl-2 positive cells in the hippocamp, frontal lobe and temporal lobe of the model group mice compared with the control. The positive cells of bax and caspase-3 are more obviously. Demi-quantitative electrophoregram of the RT-PCR product :bcl-2 gene expression is downregulated in the model than that in the normal group mice. Bax and caspase-3 are upregulated. Western blot with brain tissue protein:Bcl-2 protein expression is downregulated in the model mice than in the normal group. Bax and caspase-3 are upregulated.Conclusions:The abnormality of cervical vertebral stabilization could induce cells apoptosis in the hippocamp. The apoptotic mechanism may be that the injury could downregulate the expression of bcl-2 but upregulate bax and caspase-3.