Pharmacodynamica Studies Of Antiviral Activity Of Arbidol Hydrochloride Against Viral Keratitis

Viral Keratitis was caused of virus, most of those were herpes simplex virus and adenovirus 3.It harms visual function severely. Herpes simplex keratitis is a occoecatio oculopathy and it is at the first place. herpes simplex virus was distinguished in HSV-I and HSV-â…¡.HSV-I is the main strain infecting buccal cavity,labial part and eyes. HSK primary infection is often happened to young children. The virus could incubate ganglion nervi trigemini a long term after primary infection. It was often recurred when someone resist; resistance breakdown, just like cold, corticosteroid, immune depressant used. Matrine is one of the lightyellow sophora root extractive. It not only could resist tumour, cardiac dysrhythmia but also could antibiosis, diuresis, positive inotropic effect.It was used to cure cancer, aleucocytosis, bronchial asthma and chronic tracheitis et al.The pharmacodynamics experiment is studied byestablishing an experimental in vitro or in vivo model. of Hela cell and in vivo model of rabbit corner by herpes simplex keratitis infection. To establish an experimental in vitro model of Hela cell and prepare different concentrations,then put it into culture plate,final determain cell survival rate through observing CPE and MTT. To calculate IC50 and TI (TC50/IC50)of Mat. IC50 of Mat resist HSV-I is 2.34mg.L-1,TI is 67; IC50 of Mat resist Ad3 is 2.21mg.L-1,TI is67. The result indicate:Mat has better activity to resist on HSV-I and Ad3 comparing with virus group.In vivo, we according to master single eyes test and multiple eyes test to decide the dosage. To establish an experimental in vivo model of rabbit corner by HSV-Ior Ad3.Using different concentrates Mat to treat the lagophthalmos. The HSV-I groups take a turn for the better obviously at the sixth day. It's extent of disease dyed by fluorescein sodium deflate and infiltrate spots decrease comepared with pretherapy in the slit lamp. It is significant deviation compared with virus group (P <0.01).The result of Mat indicate: Arbidol Hydrochloride has conspicuous therapeutic action on the models of infected HSV-I,Ad3.