Functional Proteomic On Antibiotic Resistance And Protective Immunogen Of Pseudomonas Aeruginosa Outer Membrane Proteins

Pseudomonas aeruginosa, an environmentally versatile bacterium, is an opportunistic human pathogen, infecting healthy individuals rarely, but immunocompromised patients, like burn victims, AIDS-, cancer- or cystic fibrosis-patients at increased risk. This bacterium is high intrinsic resistant to most antibiotics. Thus the understanding of the resistant mechanisms and the development of vaccines are of importance in control of P. aeruginosa.This paper investigated the outer membrane proteins (OMPs) of P. aeruginosa by proteomic approach, especially immuno-proteomics. On the basis of the establishment of P. aeruginosa 2-DE map, we studied the OMPs expression difference of antibiotic-resistant strains and the immunogenic OMPs.First, we extracted the P. aeruginosa OMPs by sodium lauroyl sarcosine and identified 26 OMPs with the use of 2-DE and PMF methods. This molecular exprerssional profile of P. aeruginosa OMPs provided useful data for further studies.Then, we gained five P. aeruginosa antibiotic-resistance strains by antibiotic selection. Using the method of comparative proteomics, we found eight proteins with relate to the resistances, in which one is for erythromycin-resistance strain, two separately for chloramphenicol- and balofloxacin-resistance strains, four and five for ceftriaxone- and ampicillin- resistance, respectively.Furthermore, we screened eight immunogens of P. aeruginosa OMPs by immuno-proteomics. Then the eight immunogen genes were cloned, expressed and their recombined immunogens were used to immunize the mice. Three highly protective immunogens were achieved by challenging with P. aeruginosa to these immunized mice.