| Objective①To establish murine cytomegalovirus intrauterine infection model, to observes the female mouse pregnancy outcome and to study the effect of embryos of growth and develop intrauterine infected by murine cytomegaloviru(sMCMV);②To study pathological lesion regularity of the main organ intrauterine infected by MCMV, such as liver, brain, pulmonary, and discuss its pathogenesis mechanism.Methods①Establishing the murine intrauterine infection model by murine cytomegalovirus: MCMV Smith strains were prepared from murine embryo fibroblast (MEF) of MCMV infected mice. The sexually mature mice without MCMV infection identified by Enzyme-linked immunoabsorbent assay (ELISA) were served as the subjects, and then put the male and female in the same cages. The infection group: inject MCMV into the placentas of mice at the day 12.5 of gestation;the control groupwere injected with 3%fetal calf serum Dulbecco's Modified Eagle medium(3%FCS DMEM)at the same day . The pregnancy outcome of mother mouse and the growth and development of the infected embroys were allowed to observe until the day 18.5 of gestation or spontaneous labour;②The placental infection rate, the foetus infection rate and the main organ infection rate of foetus were detected by PCR and RT-PCR;③The morphological change in the infected placenta tissue and the main organs was observed with light microscope and transmission electron microscope.Results①Compare the infection group with the control group, the placenta infection were 63.27%(31/49) and 0.00%(0/45),P<0.001; and the embryonic infection were 32.65%(16/49) and 0.00%(0/45), P<0.001; the still-born rate were 34.69%(17/49) and 31.11%(14/45), P>0.05; the abnormal embryo rate were 22.45%(11/49) and 0.00%(0/45), P<0.001; the average fetus weight of two groups were 1.053±0.208(g) and 1.297±0.071(g), P<0.01; the average fetus body length were 25.690±2.440(mm) and 28.160±1.720(mm)P<0.01; the average fetus tail length were 10.090±0.990(mm)and 10.580±0.720(mm),P=0.03; the average fetus head circumference were 22.310±3.200(mm) and 25.390±1.170(mm), P<0.01; the average fetus brain weight were 0.055±0.011(g)and 0.069±0.001(g), P<0.01; the average ratios of the fetus brain weight to body weight were 0.052±0.002 and 0.069±0.087, P=0.27;②In the infection group, the morpholog results in light microscope showed some non-specificity inflammatory as if blood vessel dilatation, hyperemia, lymphocyte and monocyte infiltrate in placenta and liver, brain, pulmonary of fetus.In electron microscope there were some pathological lesions such as the cell swelling, the cytoplasm transparent change or vacuolated, the nuclei shrinkage, the intranuclear chromatin condensation mitochondrial enlarging, cristae disappearance or vacuolated, the Golgi's body and the endoplasmic reticulum enlarging ect;③After the placenta MCMV microinjection, both MCMV DNA and MCMV mRNA may be examined in the placenta and the embryo from the 2nd day to the 7th day, the expression of MCMV mRNA tend to be rise day by day.Conclusions①The placenta MCMV microinjection will lead infection of both placenta and embryo.The infection of MCMV appeared from the 2nd day to the 7th day and the loading doses of MCMV were increasing day by day. There was some non-specificity inflammatory in the infected organs;②The placenta microinjection MCMV would lead abnormal embryo such as microcephaly ect. There were possibly influences the growth and development of both the fetal physical constitution and the brain. |
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