The Study Of The Combined Application Of TRAIL And RANKL On Human Osteosarcoma Cells

Objective:To investigate the expression of OPG and OPGL in osteosarcoma and its significance.Methods:The expression of OPG and OPGL in human osteosarcoma tissue, osteochondroma was detected by immunohistochemistry.Results:The expressing rate of OPG was significant different between osteosarcoma and osteochondroma. The expressing rate of OPG was not significant different between osteosarcoma and osteochondroma.Conclusion:There was overexpression of OPG in human osteosarcoma. The expression of OPG may have a close correlation with the ossifying of osteosarcoma.Part two: The study of the combined application of TRAIL and RANKL on human osteosarcoma cellsObjective:To study the killing effect of the combined application of TRAIL and RANKL on human osteosarcoma cells. Methods:TRAIL and RANKL were used on the osteosarcoma cells respectively and jointly. The inhibition rates were measured by MTT assay. The apoptosis rates and caspase-3 were observed by flow cytometry. Morphological changes were observed with inverted phase contrast microscopy.Results:The apoptosis-inducing effect of the joint application was much stronger than that of the respective applications.Conclusion:Combination of TRAIL and RANKL can effectively induce the apoptosis of MG 63cell.