| Objectives: To investigate the effect of oxymatrine (OMT) on tumor necrosis factorα(TNF-α) and malondialdehyde (MDA) during myocardial ischemia reperfusion injury in rats and its protective mechanism.Methods: Male Wistar rats were anesthetized, and the left coronary artery was ligated for 30 minutes and reperfused for 180 minutes. Sixty four male Wistar rats were divided into four groups randomly (n=16): (1)sham operated group; (2)myocardial ischemia reperfusion injury(MIR) group; (3) OMT 60 mg?kg-1 treated group; (4) OMT 120 mg?kg-1 treated group. Rats were administered oxymatrine intraperitoneally five minutes before acute ischemia and lasted five minutes, then followed the steps of MIR group. TNF-αand MDA were measured before occlusion of the coronary artery, 30 minutes after ischemia and 90 minutes,180 minutes after reperfusion. TNF-αwas detected by immunohistochemistry. MDA was measured by thiobarbituric acid (TBA). 180 minutes after reperfusion, myocardial specimens were taken for ultrastructure study by using electron microscopy.Results:①A significant elevation of MDA was observed after reperfusion. Compared with MIR group, MDA in OMT groups was markedly decreased (p<0.05) after 180 minutes reperfusion. The level of MDA was no significant difference between two treated groups.②The expression of TNF-αwas increased after reperfusion. TNF-αin OMT treated groups decreased significantly compared with MIR group after 180 minutes reperfusion. But the expression of TNF-αhas nonsignificant difference in both treated groups.③Electron microscopic examination showed their pathological changes of OMT groups were ameliorated.Conclusions: Oxymatrine can prevent heart from ischemia reperfusion injury in experimental ischemia reperfusion rats. And this effect is not in a dose-dependent manner. |
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