The Study Of Effect And Mechanism Of Bax,Bcl-2 And PAP-1 In Treatment Of Severe Acute Pancreatitis

Severe acute pancreatitis (SAP) is serious harmful to human's health, and the pathogenic mechanism of SAP is still not completely clear now. There are some theories: pancreas enzymes own digestion theory; Inflammation medium theory; Pancreatic gland microcycle barrier theory; The intestinal tract bacterium transposition and"second beat"theory; The cell apoptosis theory. The cell apoptosis is a basic physiological mechanism, it can impediment necrosis and reduces the serious degree of SAP in the SAP pathogenesis.At present, there are many studies that the effect of the cell Bcl-2 protein family and the pancreatitis associated protein (PAP) in cell apoptosis mechanism, but weakly perishes in the cell in the mechanism function studies the hot spot, but the effect of them in the mechanism and treatment of SAP remain poorly defined.The medicine such as growth hormone (GH) and somatostatin (SST),the Chinese tradition medicine telramethylpyrazine (TMP) display very important function in the treatment of SAP. But they unite the medication the curative effect, they the function which displays in the treatment principle is specially to the Bcl-2 protein family and the PAP influence function research quite few; Moreover, about is the compound, has oppresses the anti-PAF active Chinese native medicine kadsurenone in to treat SAP the curative effect and in treats the function which in the mechanism displays to be also worth studying.This research is studying formerly in the foundation, through observes each kind of target to observe emphatically telramethylpyrazine and kadsurenone governs the curative effect to SAP; As well as Bax, Bcl-2 and PAP-1 three kind of gene change situations and with treatment result relations which the pancreatic gland organizes in the course of treatment.Its result discovered that, telramethylpyrazine and the sea breeze cane alkone has good treatment SAP to do; But in medicine course of treatment; The short-term (48h) Bax gene expression declines obviously, but the Bcl-2 gene and the PAP-1 gene expression obvious upward, is playing the role which the SAP acute stage suppression cell perishes weakly, but long; (72h) then situation;Has played the role which the suppression cell death promotes it to perish weakly.