| Rehmannia glutinosa Libosch. is the rhizome of Rehmannia glutinosa Libosch.It has been used in china for a long time .It is one of the four famous herds produced in Henan HuaiQin and firstly described in Shennong bencaojing and classfied as the upper class. It is often been used as a zibu herbs in china. According to the stastic,it is one of the top tenth herbs usually used in the prescriptions of traditional chinese medicine.Since 1980s, the study on the chemical components in Rehmannia glutinosa Libosch.has been developed rapidly.It includes iridoid glycosides, various polysaccharides, amino acids and a littleβ-sitosterol. Glycosides is the major part in these components and iridoid glycosides is the principal part .At present, the number of iridoid glycosides separated from Rehmannia glutinosa Libosch.has reached to 30, catalpol possesses the highest contents in all of these.In the first chapter,we summarize the study on the origin, preparation, chemical components, determination of contents and finger chromatography of Rehmannia glutinosa Libosch. in recent years.In the second chapter,we isolate and identify iridoid glycosides components from Rehmannia glutinosa Libosch. The raw crude herb is grinded and extrated by ethanol,isolated and purified by macroreticular resin and Sephadex LH-20. The structure of the compound is identified by the modern column chromatograph technology. 21 compounds are isolated from ethanol extraction and 13 compounds are identified.They are leonuride(compound 1),melittoside(compound 2),catalpol(compound 3),rehmannioside A(compound 4),rehmannioside D(compound 5),rehmannioside E(compound 6),stachyose(compound 7),raffinose(compound 8),sucrose (compound 9),daucosterol(compound 10),β-sitosterol(compound 11),D-mannitol(compound 12),aucubin(compound 13). In the third chapter,we establish the determination of the contents of leonuride and compare the change of the active components in Rehmannia glutinosa Libosch. during the course of the prepration of Rehmannia glutinosa Libosch. steamed for nine times and shined for nine times.The contents of catalpol , leonuride and rehmannioside D were determined by HPLC.Using 50% methanol as extraction solution, the herbs were extracted for 30min.The determination of the contents of three components were determined by C18 column(4.6mm×200mm,5μm). The mobile phase of catalpol was consisted of acetonitrile- 0.1% phosphoric acid–water solution (1:99),the detection wavelength was 210nm;column temperature was 25℃;the flow rate was 1ml/min.The mobile phase of leonuride and rehmannioside D was consisted of acetonitrile- water (3:97),the detection wavelength was 203nm;column temperature was 25℃;the flow rate was 1ml/min. The linear equation of catalpol is Y=284782×+7363.12,r=0.999 7.It has a good linear relationship in 0.104~1.144μg/μl. The linear equation of leonuride is Y=468717×+1409,r=0.999 7. It has a good linear relationship in 0.221~1.326μg/μl. The linear equation of rehmannioside D is Y=394650×+6273.93,r=0.999 9. It has a good linear relationship in 0.223~1.338μg/μl.The mean recovery of catalpol was 100.38% and RSD was 2.09%(n=6). The mean recovery of leonuride was 103.08% and RSD was 1.19%(n=6). The mean recovery of rehmannioside D was 100.41% and RSD was 1.41%(n=6). The method is sensitive, special and repeatable.It can be applied in the determination of the contents of Rehmannia glutinosa Libosch.The contents of three components in Rehmannia glutinosa Libosch have obvious difference.This study will provide some reference in quality control of the preparation of Rehmannia glutinosa Libosch.In the fourth chapter, by HPLC, catalpol, leonuride and rehmannioside D were used as standard to analyze and compare the common fingerprint pesks and compare the HPLC chromatography of three preparations of Rehmannia glutinosa Libosch..Results suggests common fingerprint pesks of three preparations of Rehmannia glutinosa Libosch. have obvious difference. In Conclusion, common fingerprint pesks in HPLC can be used as the important parameters of the quality control for Rehmannia glutinosa Libosch.. |
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