| Objective: To study the effect of bone mesenchymal stem cells (bMSCs) on healing of tendon-bone junction in anterior cruciate ligament (ACL) reconstructionMethods: The bMSCs of rabbits were harvested by bone marrow aspiration. bMSCs were isolated from bone marrow and purified by density centrifuge and adhered to the cultural plastic in vitro. Standard Animal model of anterior cruciate ligament (ACL) reconstruction in 18 mature New Zealand white rabbits were established and randomly classified into 2 groups. The experimental group was planted into by the composite of MSCs and fibrin glue while the control group was only operated without MSCs planting into. The rabbits were harvested at weeks 2,4, 8 postoperatively for histological examinations.Results: Control reconstructions showed mature scar tissue with some Sharpey's-like fibers spanning the tendon-bone interface at 8 weeks. The MSC-enhanced reconstructions had large areas of cartilage cells at the tendon-bone junction at 2 weeks. By 8 weeks, a mature zone of cartilage was seen gradually blending from bone into the tendon grafts.Conclusions: Tendon-bone interface transplanting bMSCs results in healing by an intervening zone of cartilage resembling the chondral enthesis of normal ACL insertions rather than collagenfibers and scar tissue. bMSC-enhanced ACL reconstructions perform significantly better than control group. |
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