Screen And Formulae Test Of Traditional Chinese Herbs Promoting Uterine Contraction

The effect of uterine reconversion and depuration after parturition was determined by contractibility of uterus, and promoting the uterine contractibi- -lity was an important measure for curing endometritis. The test was aimed at screening traditional Chinese herbs which can promote uterine contractibility by detecting the effects of several single herbs and Chinese medicinal formu- -lae on the uterine activity in vitro, which could provide references to select- -ing Chinese medicinal formulae applied to treat endometritis in clinic.Diaethylstilbestrol (1mg·kg-1) was subcutaneously injected into unpreg- -nant healthy mouse and the uterus were collected at 48h after injection. The uterine horns were longitudinally incised. Two pieces of uterine muscle, 0.8 cm long and 0.2 cm wide, were obtained and preserved in Tyrode's solution at 38.0±0.5℃. One end of the muscle was fixed by metallic hook, and the other was fixed with suture silk and connected with tono-energy transducer. In this test, Medlab biological signal collecting-and-processing system was utilized to detect the contraction effects of several herbs on the mouse uterus in vitro, while herba leonuri and neostigmine were used as the positive control groups. The results indicated that fructus aurantii immaturus, pericarpium citri reticulatae or herba menthae inhibited significantly the activity of smooth muscle of mouse uterus in vitro, and a dose-response relationship could be oberseved. Rhizoma zingiberis showed inhibitory effects on the activity of uterine smooth muscle in vitro, and a dose-response relationship could also be oberseved. Cortex magnoliae officinalis had excitatory effects on the activity of uterine smooth muscle in vitro at low concentration, while had inhibitory effects at high concentration. Semen arecae had excitatory effects on the activity of uterine smooth muscle in vitro. Rhizoma coptidis imposed excita- -tory effects on the activity of smooth mucle of uterus in vitro first, and then changed into inhibitory effects.Chinese medicinal formulae was established on the basis of observing the effects of several single herbs on mouse uterus activity in vitro, Medlab biological signal collecting-and-processing system was utilized to detect the effects of several herbal medcine formulae on the mouse uterus activity in vitro. The results indicated that mixed decoction had significant excitatory effects on the smooth muscle contraction of mouse uterus, it was extracted from that of the cortex magnoliae officinalis and semen arecae, or cortex magnoliae officinalis and rhizoma coptidis, or semen arecae and rhizoma coptidis, or cortex magnoliae officinalis, semen arecae and rhizoma coptidis, or herba menthae and cortex magnoliae officinalis, or herba menthae and semen arecae, or herba menthae and rhizoma coptidis, or herba menthae, rhizoma coptidis, and cortex magnoliae officinalis, or herba menthae, semen arecae, and rhizoma coptidis, or herba menthae, semen arecae, and cortex magnoliae officinalis, or herba menthae, rhizoma coptidis, cortex magnoliae officinalis, and semen arecae, or rhizoma zingiberis and semen arecae, or rhizoma zingiberis and rhizoma coptidis, or rhizoma zingiberis, semen arecae, and rhizoma coptidis.Lidocaine and procaine had low-grade inhibitory effects on the smooth muscle of mouse uterus. Additionally, lidocaine and fructus aurantii immatu- -rus, lidocaine and pericarpium citri reticulatae, lidocaine and rhizoma zingiberis, lidocaine and herba menthae had significant inhibitory effects on the smooth muscle contraction of mouse uterus, respectively. Lidocaine and cortex magnoliae officinalis had low-grade inhibitory effects on the smooth muscle of mouse uterus, but it was not obvious. Both of the lidocaine and semen arecae, or the lidocaine and rhizoma coptidis had significant excitatory effects on the smooth muscle of mouse uterus, but weaker than single herb's effects. Single herbs's effects on mouse uterus activity could lightly be inhibi- -ted by lidocaine in vitro.