| ObjectiveAbnormal birth weight newborn baby is that the very dangerous baby, and the abnormal birth weight baby are easy to suffer children, adult adiposity and some adult diseases. There are many factors that can lead to abnormal birth weight, among which placenta's transport function plays very important role to embryo's growth. Glucose is the most nutrient substance that the placenta transports, therefore the abnormal placenta glucose transports will affect the embryo growth. At present, the research report about the placenta glucose transporting (GLUTs) at home and abroad is less. Therefore, study on the GLUT1, GLUTs' expression law and adjusting mechanism in placenta has important significance. The reverse transcription polymerase chain reaction (RT-PCR) and Western Blot method were used in this paper to detect the GLUT1 mRNA and protein expression in the different birth weight newborn placenta of the third term. Discussed the placenta GLUT1's influence on the newborn baby's birth weight in the perinatal period.MethodsThe collection of fresh placental tissue 120 cases of different birth weight .Term delivery cases have 80 cases including without complications 20 cases, FGR 20 cases, high birth weight infants without complications and high birth weight infants who were caused by gestational diabetes mellitus 20 cases. Premature delivery cases include normal birth weight infants 20 cases and FGR 20 cases. Reveres transcription polymerase chain reaction (RT-PCR) and Western Blot method were used to assess the expression of GLUT1 mRNA and quantitative analysis of proteins in placental tissues of abnormal and normal birth weight. Statistic analysis: the results were analyzed on by SPSS software (version 13.0), there is significance difference, P > 0.05.Result1. The pregnant women's general situation.(1) Pregnant women's age, pregnancy weeks' comparisonComparing with normal group, FGR, LGA and GDM/LGA groups have no significant difference (P>0.05). There are no significant difference between SGA and LGA group (P>0.05).(2) Groups of pregnant women's body weight comparisonComparing with normal group (67.34±9.34 kg), LGA (81.02±8.45 kg) and GDM/LGA (80.67±6.59 kg) groups have a significant difference except FGR group (65.23±6.87 kg) (P< 0.05). But there is no significant difference between LGA and GDM/LGA group (P > 0.05).2. Placenta weight comparisonComparing with normal group (580.71±76.31g), FGR (418.24±60.55g), LGA (820.44±65.24g) and GDM/LGA (830.32±54.66g) groups have significant difference (P<0.05). But there is no significant difference between LGA and GDM/LGA group (P> 0.05). There is a significant difference between SGA (352.56±79.38g) and AGA (445.44±68.54g) group (P< 0.05).3. The positive expression and relative content of GLUT1 mRNA in placentaComparing with normal group (0.764±0.123), relative content of Glut1 mRNA was of FGR(0.593±0.142),LGA(0.815±0.155) and GDM/LGA(0.859±0.201) , each group has a significant difference(P<0.05); SGA group (0.504±0.086) comparing with AGA group (0.681±0.112) has a significant difference (P<0.05); LGA and GDM/LGA group have no significant difference (P >0.05). The positive expression of Glut1 in FGR group,LGA group,GDM/LGA group and normal group was 85%, 90%, 90% and 80% respectively, having no significant difference; The positive expression of GLUT1 in SGA group and AGA group was 85%, 80% respectively, without significant difference.4. The relative content of GLUT1 in placentaWestern blot analysis showed that the relative content of GLUT1 in different group was different. Comparing with normal group (65.27±9.32), the relative content of FGR group (53.13±7.78), LGA group (84.22±12.65) and GDM/LGA group (87.68±17.23) has a significant difference (P<0.05); SGA group (48.36±6.71) comparing with AGA group (61.45±7.63) has a significant difference (P<0.05); LGA group and GDM/LGA group have no significant difference (P > 0.05).ConclusionsThe placenta GLUT1 expressions of different birth weight are different. The expression of FGR group infra normal birth weigh group. The expression of high birth weight group is higher. The GLUT1 may be one of the causes that affect the fatal birth weight. |
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