Expression And Clinical Significance Of GIRK1 And GIRK2 In Cervical Cancer

ObjectivePotassium ion channels exists in most of cell membrane widely,these channels are essential for controlling membrane voltage , cytosolic pH , cell volume and appear to have a role in cell proliferation, they have massive expressions in many tumor cell. GIRK belong to inwardly rectifying potassium ion channels,there are five different subunit :GIRKl-5.The aim of this thesis was to detect the expression of GIRK1,2 mRNA in order to find their relation with cervical cancer and the prognosis by RT-PCR.Materials and MethodsMaterial: Tissue used in this study were obtaines from 60 patients with invasive cervical cancer who underwent biopsy or curative resection, and another 10 pairs cervical cancer specimens from the same patients who before and after radiochemotherapy at the First Affiliated Hospital of China Medical University. Besides, we collected 10 cases of normal cervical tissue. These tissue samples were obtained after hysterectomy because of benign uterine myoma. We collected the clinical information including pathological grades ,clinical stages, age, before and after radiochemotherapy for statistical analysis.Main agents: RNAiso Reagent, TaKaRa RNA PCR Kit(AMV)Ver3.0, Forward and reverse primer of GIRK1, GIRK2 andβ-actin ,the necessary agents of agarose gel electrophoresis.Method:RT-PCRResult judgement: GIRK1 and GIRK2 mRNA amplified by PCR,agarose gel electrophoresis,UV lamps observe results,and do semi-quantitative analysis by measuring the gray level.Statistical method:all data processed by SPSS13.0,measurement data was carried out by T-test. Differences were considered significant when P values were less than 0.05.Result1,The expression of GIRK1,2 mRNA in normal cervix and cervical cancerthe ratio of gray level of GIRK1 mRNA was 0.0254in normal cervix,1.5483 in cervical cancer,there were significant differences(P<0.01). the ratio of gray level of GIRK2 mRNA was 0.3865 in normal cervix,1.8339 in cervical cancer,there were significant differences(P<0.01).2,Correlation between the expression of GIRK1,2 mRNA and clinicopathologic materials in patients with cervical cancerThe ratio of gray level of GIRK1 mRNA was 1.8638 in the poor differentiation group,higher than that of the middle and high differentiation group 0.9857, 0.8564 (P<0.05). the ratio of gray level of GIRK1 mRNA was 0.9894 in stages I～II ,1.9752 in stages III～IV,there were significant differences(P<0.01). The ratio of gray level of GIRK2 mRNA was 2.0539 in the poor differentiation group,higher than that of the middle and high differentiation group 0.8611,1.2250(P<0.01). the ratio of gray level of GIRK2 mRNA was 1.1872 in stages I～11,2.5321 in stages III～IV,there were significant differences(P<0.05). The expression of GIRK1 and GIRK2mRNA have no significant relation with age(P>0.05).3,The expression of GIRK1,2 mRNA in the same patient with cervical cancer before and after radio-chemotherapyGIRK1 mRNA was 1.3710 before radiotherapy and chemotherapy,0.2939 after radiotherapy and chemotherapy,they have significant differences(P<0.01). GIRK2 mRNA was 1.9581 before radiotherapy and chemotherapy,0.5704 after radiotherapy and chemotherapy,they have significant differences(P<0.01).Conclusion1,GIRK1 and GIRK2 mRNA participate in the proliferation of cervical cancer cell. 2,GIRK1 and GIRK2 mRNA have a positive correlation with clinical stages and pathological grades,so they may be correlated with the malignant progression and prognosis of cervical cancer.3,The expression of GIRK1 and GIRK2 mRNA in cervical cancer decrease after radiotherapy and chemotherapy.