Protective Effects Of Hormone Replacement Therapy On The Changes Of Impaired Genital System Of Female Rat Induced By Tripterygium Wilfordii Polyglycosidium

Background and objective TWP (tripterygium wilfordii polyglycosidium) not only retains the action of immune suppression but also removes many toxicity compositions as the extract of tripterygium wilfordii Hook f. It is applied in clinic generally including renal glomerular disease,systemic lupus erythematosus,rheumatoid arthritis and so on but it's adverse effects confine clinicial application especially its disadvantage of genital system. Estrogen- Progestogen sequential replacement therapy is often applied in the patient whose ovary was injuried because of in critique age or operation or radiotherapy and chems in clinic. It is reported that Fas-FasL system found on granulosa cell of the majority of living being ovary including the human being and rat participates apoptosis of granulosa cell. The objective of this study is to observe the effect of Estrogen- Progestogen sequential therapy on the changes of female rat genital system induced by tripterygiumw ilfordiipo lyglycosidium and to approach the possible mechanism on the basis of the expression of Fas and FasL.Methods Sixty female rats with normal oestrus cycle were randomly divided into three groups: control group, TWP group, TWP and estrogen combined with progestogen replacement therapy periodical group(TWP+HRT). these three-group rats were feeded in normal sodium, TWP and TWP+HRT differently. The dose of TWP was tevlve mg per mg per day and the dircetion of HRT was that Estradiol Valerate Tabletes after three-day dosis was used one day combining with Medroxyprogesterone Acetate Tablets then these were stop using one day . HRT was always repeated to use by time of tevlve weeks. These rats were put to death after tevlve weeks. The weight of the ovary and uterus were measured by electronic balance. Oestrus cycle was observed by Pap staining. The serum level of E₂ (estrogen),FSH (follicle-stimulating hormone) and LH(luteinizing hormone) were detected by RIA. Pathological changes of the ovary and uterus were observed by light microscope according to hematoxylin and eosin stain. The expression of Fas and FasLmRNA were detected by RT-PCR on granulosa cell.Results 1. Clinical and general parameters compared with control group, oestrus cycle was disorder or extension, estrogen level and the weight of the ovary and uterus lowered (PO.05) ,the level of FSH and LH increased (P<0.05) in TWP group. No obvious change was found in TWP+HRT group(P>0.05).2. pathologic morphology Worse-growing follicle, degenerating corpus luteum and thinner endometrium with diminishing uterine gland were found in TWP group compared with control group. But the ovary and uterus of TWP+HRT group rat were similar to the rat of control group.3. the expression of Fas and FasLmRNA on granulosa cell This study indicated Fas and FasLmRNA can also express on granulosa cell of the ovary. But No difference of the expression of FasLmRNA was found in these three groups(P>0.05). FasmRNA cann't be detected because of its quick apoptosis.Conclusion TWP combined with HRT can decrease the toxic and side effects of TWP on the genital system of female rat and protect the ovary.