| Background Systemic lupus erythematosus(SLE) is a complex systemic autoimmune disease characterized by a diverse array of autoantibody production, complement activation,immune complex deposition,and tissue and organ damage, signifying that genetic and environmental factors are involved in disease development andpathogenesis,their interaction is not known.Recently findings suggesting a role for the EBV have been put forward.In addition it is most likely that other factors such as environmental hazards such as smoking and pesticides may play a role.Well known is the fact that several drugs do induce lupus in susceptible individuals,but ethnic and genetic heterogeneity contributes to the complexity in its clinical presentation. Therefore,to explore polymorphisms of the gene correlation with SLE have become hot spot of research.Osteopontin(OPN) is also known as early T-lymphocyte activation-1 (Eta-1),which is expressed 19-fold higher in T-helper type 1(Th1)-cells compared with Th2-Cells,OPN has been defined as a Th1 cytokine.It enhances a Th1 response by inducing macrophages to produce IL-12 and INF-γ.OPN may influence autoimmune diseases through its immunoregulatory effects,enhancing the proinflammatory Th1 cell response and inhibiting the Th2 responses.Objective:To Detect the relevance of the polymorphism of OPN and its plasma OPN levels with the incidence and clinical manifestation of SLE.Methods:143 cases of SLE patients(age:39.4±12.1 years;female / male:134 / 9) in line with the United States ACR1997 classification criteria,and 97 cases(age:34.4± 9.5 years;female / male:87/10) healthy Physical examination participants as the control group,using real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) to test the polymorphism of OPN gene C707T,and then compared the genotype distribution and allele frequencies between different groups.The plasma OPN level of 68 cases of SLE patients(age:38.2±11.7 years;female / male:64 / 4), 35 cases of rheumatoid arthritis(RA) patients(age:43.6±18.9 years;female / male: 27 / 8),34 cases of ankylosing spondylitis(AS) patients(age:30.6±9.5 years;female /male:6 / 28) and 36 cases of the normal control group(age:36.4±10.2 years;female / male:33 / 3) are detected by ELISA for the comparison of the discrepancies among different groups,while the SLEDAI score and laboratory indicators of the SLE patients during the blood collection were recorded and analyzed for their relationship with the plasma OPN level.Results:1.these was no significant difference in genotypic frequencies and allelic frequencies of OPN 707C/T between SLE patients and the controls(P＞0.05).2.the genotypic frequencies and allelic frequencies of OPN 707C/T of LN group were obviously different from those of the control group(P＜0.05).3.The plasma level of OPN was significantly higher in SLE patients than in the healthy control group(4.5±2.0ng/ml vs 1.6±0.7ng/ml,P＜0.001).In SLE patients,the plasma level of OPN was significantly higher with active disease than with inactive disease(5.3±2.0ng/ml vs 3.4±1.3ng/ml,P＜0.001),in patients with renal damage than in patients without renal damage(5.8±2.1ng/ml vs 3.5±1.3ng/ml,P＜0.001).in patients with pneumono-interstitial disease,with gastrointestinal tract disease,with pericarditic disease than in 30 patients without organ damage(4.8±1.2ng/ml,6.3±1.4ng/ml,5.4±2.6ng/ml vs 3.5±1.3ng/ml,P＜0.05),in patients with positive anticardiolipin antibody than in patients with negative anticardiolipin antibody(5.3±2.4ng/ml vs 4.2±1.7 ng/ml,P＜0.05).4.The plasma level of OPN correlated positively and significantly with SLEDAI score(r=0.523,P＜0.001) and 24-hour urine protein excretion (r=0.403,P=0.001) in all SLE patients,but correlated negatively and significantly with serum C3 level(r=-0.398,P=0.001).There was not correlation between the plasma level of OPN and anti-dsDNA antibody,Sm antibody,erythrocyte sedimentation rate, globulin,immunoglobulin G,immunoglobulin A,immunoglobulin M,arthritis(P＞0.05).5.The plasma level of OPN was higher in SLE,RA,AS patients than in the healthy control group(P＜0.05),in SLE patients than in RA,AS patients(P＜0.001),there was not correlation between the plasma level of OPN in RA patients and in AS patients(P＞0.05).Conclusion:1.Our data suggest that the polymorphism of the OPN gene 707 C / T has nothing to do with the susceptibility of SLE disease among the Han nationality;while C707T polymorphism may be relevant to the occurrence of LN;2.The OPN levels of LN patients were significantly increased,which probably because of the existence of C707T polymorphism which promoted the OPN plasma levels of LN patients,leading to the aggravation of the disease.More significant OPN increment can be seen in active SLE patients and those with kidney,lung and other organ damages,which can be used clinically as an indicator for the observation of disease activity and organ damage.3.The OPN levels of SLE,RA and AS patients were significantly increased, thus OPN may be engaged as an important immune inflammatory factor,participating in the pathogenesis of rheumatoid immune diseases such as SLE,and being related with the diseases activity. |
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