The Study Of Anti-cancer Gene Vaccine And Corresponding Transgenic Model

Malignant tumor is a fatal killer threatening uman's health and life seriously in nowadays world.With the improvement of the standard of living and the changes of lifestyle, as well as the pollution of environment and other factors,tumor incidence has significantly being increasing.as tumor vaccine playing an important role on the treatment of malignant tumor, more and more attention has been focused on immune therapy research.In our research, we established a human tumor antigen surviving and hCGβeukaryotic expression vector which have become two transgenic positive cell line. We choosed bi-eukaryotic expression carrier called pIRESneo, which has a total of two-gene expression of brain myocardial Yan virus (ECMV) of the internal ribosome entry site (IRES) at the same linked to the promoter under the control of tumor gene and neomycin resistance gene Neo+, so that the process of translation from the ribosomes can enter different locations while two translation protein. In addition, neo-gene contains the amino-glycoside antibiotic (G418) resistance protein, through the G418 selection, almost all the survival cells of cloning are able to express the purpose gene of transfer, which greatly reduces the necessity of filtering a function Monoclonal antibody from the many in the drug fast cloning. Hence, not only saved the time but also reduced the workload. In this article a kind of cells with stable expression of the aim gene has been established eventually, and providing materials for research in establishing animal models of targeting treatment of breast cancer.At the same time, we construct an anti-tumor multi-target DNA vaccine, which targeting the core protein CTP-37 of the chorionic gonadotropin hCGβchain. Then we insert CTP-37 gene fragment of Human and monkey's into upstream of the IRES in the pVAX1-IRES-GM/B7 carrier, thus successfully constructed a Multi-target union efficiency anti-tumor gene vaccine. Our research provides a foundation for further study of vaccine function identification in this animal model.