Study On The Relationship Between ENOS Gene Polymorphism And Type 2 Diabetes With Hypertension In The People Of The Han Nationality Of Jilin Region

Objective:To investigate the association between the single nucleitide polymorphism in exon 7(G894T) and the insert/deletion polymorphism of variable number of tandem repeat in intron 4(4a/b) of the endothelial nitric oxide synthase (eNOS) and type 2 diabetic patiens with hypertension in the Han population in jilin region.Methods: This research is divided into research object totally 3 sets. The first group(A group) is the diabetic patiens without hypertension including 45 persons(17 male, 28 female, average age was 48士8.54); The second group(B group) is the diabetic patiens with hypertension including 35 persons(13 male, 22 female, average age was 51士7.46); The second group(NC group) is matched control including 36 persons(17male, 18 female, average age was 47士8.44);Carry on 3 sets to check contrast control study. The next step is abstracting template DNA, adopt the American Promega company produce of the DNA extract kit quickly abstracting person`s white cell DNA of peripheral blood. The G894T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.The 4a/b polymorphism was determined by polymerase chain reaction-variable number of tandem repeat (PCR-VNTR) analysis.the last step is 2% agarose gel electrophoresis was performed,the results were observed under ultraviolet light. Result: (1)Hardy-Weinburg inherit quilibrium laws examination result is that each genotype frequency attains heredity balance and have a community representative . (2)The frequency of T allele and TG genetype at exon 7 of eNOS were significantly higher in the DM with hypertension group than that in matched control group;but there was not have no obvious difference between DM without hypertension and DM with hypertension.the result of DM without hypertension and matched control is the same. (3)The frequency of a allele at introns 4 of eNOS was significantly higher in DM with hypertension group and DM without hypertension than in matched control group. there was not have no obvious difference between DM without hypertension and DM with hypertension.Conclusion: ( 1 ) Hardy-Weinburg inherit quilibrium laws examination result is that each genotype frequency attains heredity balance and have a community representative .(2)the 894G→T polymorphism at exon 7 of eNOS gene may be a predisposal factor for diabetic with hypertension in jilin.T allele is related to diabetic with hypertension in type-2 diabetes mellitus(3)a allele of the 4a/b polymorphism at introns 4 of eNOS gene is related to diabetic.