Study On Construction And Immunity Of Nucleic Acid Vaccine Including SFV RNA Replicon

We construct two kinds of SFV RNA replicon-based nucleic acid vaccines pIRSFV-HN and pIRSFV-Apoptin which is correct identificated by restricted enzyme digestion. The results of RT-PCR,Western blotting and IFA indicate that the exogenous gene could express in HepG-2 tumor cell. The in vitro tumor suppression was determined by AO/EB staining , DAPI staining and MTT staining and the results of AO/EB staining , DAPI staining and MTT staining indicate that pIRSFV-HN and pIRSFV-Apoptin conld kill HepG-2 tumor cell effectively, the maximum mortality rate are 55.43% and 53.55%. C57BL/6 mice model bearing H22 hepatoma was copyed and we inject pIRSFV-HN and pIRSFV-Apoptin in the tumor. The in vivo tumor suppression was determined by CTL and detecting the level of Th1 and Th2 cell factors and the results indicate that pIRSFV-HN and pIRSFV-Apoptin could induce intensive CTL, and precipitate immunization to TH1 tendency.