| Objective The model of Kirshner wire fixation for mouse tibia fracture was established by the standard methods. Mouse VEGF was used in the experimental group following intraperitoneal injection ,and the control group was only made animal model for fracture. We had taken the X-ray films ,sliced on fracture ,and observed with light microscope in different time after operation .the purpose of this study is to observe the effect of VEGF in fracture healing and to investigate the influence of VEGF in fracture healing.Methods 42 healthy male mice were randomly divided into 2 groups, each with 21. We made incision at right knee joint.The tibial intercondylar was bored by 0.7 mm syringe needles . The axenic Kirshner wire which is about 0.35 mm in diameter, 1.2 cm length was inserted along the medullary cavity direction . After muscle tissue was dissected bluntly on the tibia,We broke the tibia transversely and closed the skin with suture. VEGF was used in the experimental group following intraperitoneal injection, 5 ng of each, twice a day, in two weeks. the control group was only made animal model for fracture. Seven mice were sacrificed in each group at 1,2,3 weeks after the operation respectively. Two groups were taken X-ray after being taken out right tibia for contrastive and dynamic observation on bone callus growth and fracture healing. After the removal of Kirshner wire, we sliced on fracture and conducted HE staining for observation on new capillaries, trabeculae of bone, cartilage cells, bone cells, and other cells as well as histological changes .According to cartilage ossification rate we made a statistical analysis for comparative study of two groups of fracture healing.Results 1.X-ray observations, after three weeks the experimental group has clearly bone callus, local thickening, higher bone density, fracture lines disappear, fracture healing. Among the control group one has continuity of bone callus , five have bone callus , but the fracture line are still visible, and the rest non-bone callus growth.2.histological observation, after three weeks the experimental group , both internal and external callus altered, plastic building further improved, breaking end connected by callus formed by trabeculae of bone .The wounded limbs repaired completely ,fracture disappeared and marrow cavity unobstructed again. Among the control group one breaking end connected by callus formed by trabeculae of bone, four were visible bone callus but their fracture line were clear, and two breaking end were still evident, not healing. 3.statistical analysis, in the first week after operation, there were no significant differences in the proportions of cartilage ossification between the two groups(P> 0.05) .In the second and third weeks after operation, there were significant differences between them(P <0.01).Conclusion The findings demonstrate that Mouse VEGF can promote fracture healing through the promotion of growth in blood vessel in breaking end and soft tissue , increase in blood flow , and promotion of the proliferation of endothelial cells, regulation on osteoclast activity, participation in the cartilage of maturity and absorption, and inducement to bone formation.Significance VEGF plays an important role in bone repair, which have acquired broad agreement .While scholars had undertaken extensive research on VEGF treatment for fractures, and achieved a breakthrough continuously, but there has been long way for VEGF to study in clinical use safely and effectively. Along with the VEGF research , deep understanding of the mechanism for fracture healing and molecular biology as well as gene technology improvement and development , orthopaedic fields such as fractures, bone defects, healing process during allogeneic bone graft is expected to be shortened due to the commercial applications of VEGF. The deep VEGF research will surely opened up broad prospects for this treatment of the diseases. |
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