The Sensitizing Enhancement Effect Of Compound Kushen Injection On The Irradiation To Human Cervical Carcinoma Cell(Hela)

Objective: To investigate the cytotoxicity and radiation sensitizing enhancement effect of compound Kushen injection on Hela cells in vitro.Methods: The cytotoxicity of different concentration of compound Kushen injection on Hela cells was observed, and IC10 was calculated by MTT method. The Hela cells were exposured in IC10 of compound Kushen injection after 24 hours and 48 hours. The two groups and radiation alone group received 6MV X-ray ,their absorption dose are 0Gy,1Gy,2Gy,4 Gy,6Gy,8Gy,10Gy respectively. Colony forming assay was used for survival fraction analysis. Single-hit multi-target model was used to plot survival curve. Graphpad statistics software is used to analysis parameter .Determined the values of D0 and Dq of three groups, calculated sensitization enhancement ratio(SER) of (R+T)1 group and (R+T)2 group .Result:1 Compound Kushen injection has the proliferation inhibitory action to the Hela cells. The results of MTT also assumes positive correlation. The value of IC10 was 4.330μl∕mL.2 Single-hit multi-targeted model fitting the cell survival curve demonstrated that the value of D0 of (R+T)1 group and (R+T)2 group decreased. From control group′s 1.36Gy falls to the group pretreatment with Mat for 24 hours and the group pretreatment with Mat for 48 hours 1.05Gy and 0.77Gy respectively. The two study groups shoulder area become narrowing. The values of Dq also decreased from control group's 1.72 Gy falls to(R+T)1group and (R+T)2 group 1.48 Gy and 1.4Gy respectively. The values of SER of (R+T)1 group and (R+T)2 group are 1.30 and 1 .76 respectively。Conclusions:1 Compound Kushen injection has been shown obvious cytotoxicity and it assumes positive correlation .2 It shows compound Kushen injection has somehow radiosensitizing effects on human cervical carcinoma cells in vitro .The group of (R+T)1 is more effect than (R+T)2 group.