| Objective: To explore the mechanism of FXR in ICP enterohepatic circulation by building the rat model of intrahepatic cholestasis of pregnancy(ICP) induced by estradiol benzoate(EB) and detecting the expression of farnesoid X receptor(FXR) and IBABP (protein and mRNA) in ileum。Methods: 30 SD pregnant rats for 15 days were randomly divided into two groups: treatment group, control group, and given respectively the subcutaneously injection of estradiol benzoate(EB)at a dose of 2.5 mg kg-1d-1or appropriate volume of isotonic Na chloride (NS) for 5 days . Venous blood was sampled for the measurement of serum ALT,AST,ALP,TBA,TBIL,DBIL level for twice(before given medication, before delivery). Liver and ileum were taken for histological analysis with light microscope by HE staining after delivery; some physiological indexs of the fetal rats and datas placental weight has been measured and been entering into statistic. The expression of FXR/IBABP in ileum was analyzed by immunohistochemical method (SP) and The expression of FXR/IBABP mRNA was analyzed by RT-PCR.Result: The serum levels of ALT,AST,ALP,TBA,TBIL,DBIL in the experiment group rats increased significiantly(p<0.05), control group had no changes (p>0.05); The physiological indexs of fetal rats and datas of placental weight were analyzed,and results indicated that control group was better than experiment group(p<0.05); The pathological changes showed intrahepatic cholestasis in pregnant rats in experiment group In experiment group, the degrees of FXR and FXR mRNA in the ileum were significantly higher (p<0.05),and the IBABP and IBABP mRNA were higher too (p<0.05) contrast with control group.Conclusion: Estradiol benzoate is the best candidate for inducing animal model of ICP. Because of cholestasis induced by Estradiol benzoate, FXR and IBABP (protein and mRNA) increase in ileum, which transport more bile acids into portal vein。This may be one of the mechanism of intrahepatic cholestasis of pregnancy. |
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