The Study Of Ultrasound Mediated Taxol Carrying Liposome Microbbles Treatment Of Ovarian Cancer Cell Line

PARTⅠTAXOL CARRYING LIPOSOME MICROBUBBLES SECT ULTRSONIC RADIATION DAMAGE PARAMETERS AND THE CONCENTRATION OF PACLITAXEL IN OVARIAN CANCER CELLS IN VITRO TREATMENTObjective : To study the ultrasonic radiation parameters of destructing paclitaxel-carrying lipisome microbubbles(PLM) and paclitaxel treatment concentration of ovarian carcinoma cells SKOV3 in vitro.Methods : The inhibitory effects of different dosages of paclitaxel on SKOV3 cells proliferation and the acute toxic effects of ultrasound combined PLM on SKOV3 cells was detected by MTT assay.Results : Certain concentrations of paclitaxel and ultrasound alone both have significant toxic effects on SKOV3 cells. PLM could be destructed with an acoustic intensity of 0.25W/cm2 and the irradiation time of 30 S. When the acoustic intensity was fixed in 0.5W/cm2, and the time of ultrasound radiation on PLM were 10 S, 20 S and 30 S, the survival rates of cells were determined respectively as 92.01%, 84.91% and 80.17%. The proliferation inhibition ratio of SKOV3 cells in the 72nd hour were determined as 30.05%, 37.69%, 56.46% and 66.59% after paclitaxel treatment with different concentrations of 10-8, 10-7, 10-6 and 10-5mol/L, respectively. And the inhibition ratio of SKOV3 cells in the 24th hour, 48th hour and 72nd hour were respectively 9.82%, 23.12% and 30.05% when the palictaxel concentration was fixed in 10-8mol/L. The inhibition effect was positively related with the time and concentration of drug treatment, which showed an obvious dose-response time relationship.Conclusion: paclitaxel could inhibit SKOV3 cells in vitro. There is a little acute toxic effects on SKOV3 cells using ultrasound combined PLM method. Suitable ultrasonic irradiation parameters is 0.5W/cm2×30 S, and paclitaxel dosage is 10-6mol/L. PARTⅡTHE EFFECT OF ULTRASOUND COMBINED PACLITAXEL CARRYING LIPOSOME MICROBUBBLES ON OVARIAN CARCINOMA CELL PROLIFERATION AND APOPTOSIS AND CJICobjective: To study the effects of ultrasound combined paclitaxel-carrying liposome microbubbles(PLM) method on SKOV3 cell proliferation, apoptosis and gap junctional intercellular communication(GJIC).Methods: SKOV3 cell proliferation situation was determined by MTT assay after ultrasound combined PLM treatment. SKOV3 cell apoptosis was detected by flow cytometry(FCM) and transmission electron microscope(TEM). GJIC was observed by laser confocal microscopy before and after ultrasound and PLM treatment.Results: Ultrasound combined PLM, paclitaxel alone and ultrasound combined PLM all had significant inhibition effects on SKOV3 cell proliferation. However, among the three groups, the effects of ultrasound combined PLM group was the strongest(P﹤0.010) . And PLM alone with the concentration equal with the first group had no significant effects on cells. Apoptotic bodies were observed by TEM in the ultrasound combined PLM group, apoptotic cells were observed in the ultrasound combined paclitaxel group, and the early apoptotic cells were observed in the paclitaxel alone group. Cell shape in the contrast group and the PLM alone group were both normal. The apoptosis rates of cells determined by FCM were respectively (1.54±0.15)% in the contrast group, (6.26±0.26)% in the PLM alone group, (11.06±2.87)% in paclitaxel alone group, (16.95±0.70)% in the ultrasound combined paclitaxel group and (26.87±0.56)% in the ultrasound combined PLM group. SKOV3 cell GJIC function was up regulated significantly among all the groups.Conclusion : Ultrasound combined PLM method could not only inhibit SKOV3 cell proliferation, but also induce apoptosis and up regulate cell GJIC function.