The Relevance Research Between Lung Tumor's Vessel Three Dimensional Reconstruction And CD34 Marked Capillaries Density

Objective:1. The research aims to explore the three dimensional reconstruction performances of the lung tumor blood's multi-layered screw CT(MSCT) and the characteristics of the CD34 marked tumor micro-vessel density(MVD).Also the relevance between them is analyzed.2. Comparative analysis on lung tumor on lung tumor surgical operation apecimen and filament mirror biopsy specimen CD34 marked capillaries density is conducted, establishing convenient and accurate evaluation system, which provides help for the clinical involvement treatment.Method:1.the research applies the weidner method in 40 instances in chongqing medical college. These subjects are patients who have been provided with lungs malignant tumor parallel surgical operation and filament mirror biopsy treatment CD34 mark micro-vessel counting measurement is conducted on these specimens .Then the tumor blood vessel not spot area (obvious dyeing ) is checked out under minor-multiple mirror .Again counting measurement under the power mirror dyeing blood vessel quantity is conducted, employing 3 field of vision the capillaries, the field of vision area is 0.443mm2.at last ,the average MVD value is counted.2.The MSCT three dimensional reconstruction :adopting GE-slight speed 64 row of screw CT, 80ml~90ml iodine sea mellow high pressure injection image formation , speed 3.0~4.0ml/s, each cross section scans 1,level thick 0.625~1.25mm, level spacing 0.5mm.in progressive scanning seeks each layer which the tumor dyes (arteria bronchialis time appearance time section image stratification plane)and the value area which blood vessel density CT value 70~301HU ,the clinical three dimensional, 3D- VR blood vessel reconstruction superimposition pathological change area and the vasculosa area, the color parameter has being adjusted until the gain may satisfy the gain blood vessel counting clinical picture material effect. Observations on the 1cm2 picture area scope continuously, accordingly the precision reaches 1.0mm to be possible to integrate the blood vessel counting.Results:1.the average micro-vessel density of the normal tissues of the 40 patients (17.23±6.52)/HP, and the average micro-vessel density of the lung tumor mag maligheat is (27.74±3.94)/HP, obviously, the lung tumor group is bigger than the normal tissue MVD(P<0.01),In the 40 surgery specimen ,the scale cancer 28 examples and the gland cancer 9 examples, and all of them are arteria bronchialis blood supply ,also 12 examples'lung tumor enhancement CT reconstruction image displays that tumor lump body has in and out the pulmonary artery to pass through , the encystations or to vanish nearby the tumor , no pulmonary artery becomes thick or enters.2, Applying MSCT in normal and the supply tumor arteria bronchialis initial sector inside diameter observation ,the supply tumor arteria bronchialis initial sector inside diameter is normal increasing 1~2mm(to be able to observe accurately is in doubt ,evidence is few; further observation is needed and exploration is followed), there is significant difference between the tissue arteria bronchialis of the normal and the central committee lung cancer and the periphery lung cancer patients(P<0.01) 3, Straight line correlation analysis is conducted on The surgical operation lung malignant tumor specimen MSCT blood vessel counting and corresponding surgery specimen CD34 marks the MVD determination.Both are bestowed with positive correlation (r=0.754, P=0.000) 4, The lung tumor surgical department excises the surgery specimen CD34 based on the lung tumor surgical specimen CD34 the micro-vessel density determination of lung cancer organization is 27.74±3.94 strip /HP, the micro-vessel density determination of the lung cancer organization 26.90±2.76 strips/HP. which is based on the filament mirror pliers with the tumor organization specimen CD34. Then independent sample T-test is conducted ,non-statistics significance exists.5, The straight line correlation analysis is conducted on the lung tumor surgical specimen and the filament mirror pliers specimen tumor organization specimen CD34 capillaries density determination. Both are endowed with positive correlation (r=0.812, P=0.000) Conclusion:In summary, the MVD quantity and the tumor malignant degree is related. Filament mirror biopsy specimen and surgical operation specimen MVD are positively related with each other present correlation. And exploration on filament mirror substitution lung organization biopsy can be used directly in clinical histology examination or not. because most of clinical cases are mid or late level illness, while surgical operation only could cure part .Another part of cases which even widespread shift surgery cannot be excised as well as some feeble cases cannot be biopsy of illness can be treated with the MSCT three dimensional reconstruction pathology method or the MVD examination to diagnose the good or malignancy of the tumor ,this operation is based on the positive correlation between MSCT group blood vessel counting and the tumor malignant degree. Specially high speed MSCT SSD, VR, may determine the tumor in the clinical practice reconstruction image which displays the blood supply characteristics, the great help has been provided through the tumor blood supply characteristic value on the tumor involvement treatment medicine supplying with best spot choice and the treatment scope determination.