| Objective: To investigate the heterogeneity of chemosensitivity and feasibility of the ATP-tumor chemosensitivity assay (ATP-TCA) applied in chemotherapy and evaluate sensitive rate of specimens of gastric carcinoma to sixteen cytotoxic drugs.Methods: An ATP-TCA were used to determine the effect of 16 single cytotoxic drugs in freshly-taken surgical specimens from 44 patients with gastric carcinoma, some cytotoxic drugs as follows: paclitaxel(TAX), gemcitabine (GEM), doxorubicin(ADM), cisplatin(DDP), fluoruracil(5-FU),et cetera.Results: (1) Evaluative test results were achieved in 43 of 44 specimens, the evaluable rate of ATP-TCA was 97.73%. (2)The most active single drugs in the assay was identified as 5-fluorouracil(5-FU), paclitaxel(PTX), pirarubicin(THP), and vinorelbine(NVB) in order. (3)5-fluorouracil was found to be the most effective drug, but drugs regularly used such as cisplatin(DDP), carboplatin(CBP), and etopside(VP-16) were just weakly active or resistive.Conclusion: There were considerable differences and heterogeneity of the in vitro chemosensitivity between individuals in gastric carcinoma. The use of the ATP-TCA provides a method of selecting appropriate anti-cancer drugs in gastric carcinoma, it is a sensitive, reliable and standardized method for tumor chemosensitivity testing, it deserved to study further for screening anticancer agents in chemotherapy... |
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