Changes Of IL-6 And IL-8 In The Artery Blood During Pulmonary Operation With One-lung Ventilation

Objective To observe the changes of IL-6,IL-8 in the arterial blood with one lung ventilation in pulmonary lobectomy.To investigate the influence on the lung inflammatory during one-lung ventilation.Methods Twenty ASAⅠ～Ⅱpatients scheduled for elective pulmonary lobectomy were randomly divided into two groups of ten each,namely one-lung group and double-lung group. There were no obvious difference in the methods of anesthesia and test facility between two groups.One-lung group were immediately practiced one-lung ventilation after anesthesia induction and double lung ventilation immediately after the surgery in the chest were over.Two milliliters of blood samples were obtained from radial artery for Hct before anesthesia(T₀),30 minutes after mechanical ventilation(T₁),one hour after mechanical ventilation(T₂),two hours after mechanical ventilation(T₃),one hour after completing surgery in the chest (T₄),two hours after completing surgery in the chest(T₅),24 hours from operation(T₆),48 hours from operation(T₇).Three mils artery blood samples were collected on above-mentioned time points for determination of blood concentrations of IL-6 and IL-8 which were measured by ELISA.Result(1)IL-6: compared with the time point of before anesthesia(T₀),two groups all began to increase with one hour after mechanical ventilation(T₂)(P＜0.01),reached climax two hours after completing surgery in the chest(T₅),decreased gradually at 24 hours from operation(T₆),could not restore to the level before anesthesia(T₀)(P＜0.05).One-lung group were higher than those in double lung group at time points of T₂,T₃,T₄,Y₅,Y₆.(P＜0.05)(2)IL-8:compared with the time point of before anesthesia(T₀),two groups with all began to increase with one hour after mechanical ventilation(T₂)(P＜0.05),reached climax two hours after completing surgery in the chest(T₅),decreased gradually at 24 hours from operation(T₆),could not restore to the level before anesthesia(T₀)(P＜0.05).One-lung group were significantly higher than those in double lung group at time points of T₃,T₄,T₅,T₆(P＜0.05).Conclusion(1) Mechanical ventilation consist of one-lung ventilation and double-lung ventilation resulted in increase of IL-6 and IL-8,which implied ventilation resulted inflammatory reaction.(2)After a period of ventilation the level of IL-6 and IL-8 in One-lung group were higher than those in double lung group.The inflammatory reaction in one-lung ventilation were more serious than double lung ventilation.(3)The level of IL-6 and IL-8 in One-lung group at 48 hours from operation were almost stepped down to the level of double lung group.So if we could control the time of one-lung ventilation,the inflammatory reaction from one-lung ventilation was not more serious than double lung ventilation anesthesia after a period of postoperative recovery.