Effects Of Heated Cooking Oil Products On Drosophila Melanogaster's Longevity And The Moleculer Toxicological Study

Objective: The purpose of these experiments is to investigate the toxicological effects of the products of heated cooking oil on Drosophila Melanogaster.①To study the content of phthalic acid ester in edible oil and it's heated products.②To learn the effect of the products of heated cooking oil on the lifespan of Drosophila Melanogaster and the activity of total-SOD, CuZn-SOD and the concentrantion of MDA.③To study the impact of the products of heated cooking oil on the gene expression of CuZn-SOD, Mn-SOD and CAT.④To observe the change of reproductive organs of Drosophila Melanogaster.⑤To learn the effect of the products of heated cooking oil on Drosophila Melanogaster head's gene expression profile. Methods: DBP and DEHP in cooking oil and it's heated products were determined by Gas Chromatograph-Mass Spectrometry (GC-MS) with selected ion recording (SIR) technique.②Longevity test was carried out to find the influence of products of heated cooking oil on Drosophila's lifespan.③We detected the activity of total-SOD and the concentration of MDA by biochemical analysis methods, in order to research the relation between effects of products of heated cooking oil on the antioxidant system and aging in Drosophila.④we studied the characterization of reproductive and developmental toxicity of the products of heated cooking oil on Drosophila by pathology slicing.⑤RT-PCR was an effective technique to display the alterations in the gene expression of Cu, Zn-SOD,Mn-SOD and CAT in Drosophila's head after the used cooking oil exposure. Moreover.⑥we get the gene-expressed profile of used cooking oil-induced Drosophila Melanogaster's head tissue by gene chip. Results:①Amounts of DBP in unheated cooking oil, used cooking oil and cooking oil fume were found in the ranges of no detected (ND)～0.78mg/L, (ND)～0.86mg/L and 0.04～11.94mg/L, the amounts of DEHP were (ND)～2.11mg/L,(ND)～2.25mg/L and 2.57～95.49mg/L respectively.②The LT50 of test group was lower than the control group, shortened by 10 days～37 days and 1 days～24 days respectively; MLS was shortened by 9days～30 days and 2days～23 days respectively, MMLS was shortened respectively, MMLS was shortened by 16days～44 days and 5 days -33 days respectively; Except the male 0.67% group of cooking oil fume, the mean life-span and maximal mean life-span of test groups were significantly shortened (P＜0.05 or P＜0.01).③Our results showed a time-dependently decreasing of the SOD activity and increasing MDA content of the exposure groups.④The products of heated cooking oil damaged the reproductive organs of male Drosophila.⑤In the molecular level, used cooking oil can suppress the gene expression of CAT in male Drosophila and Mn-SOD in both male and female (P＜0.05).⑥We found 154 down regulated genes and 93 up regulated gene. These genes are regulated with signal transduction, reproductive founction, growth and immunity defense. Conclusion: Unused cooking oil have been polluted by DOP and DEHP through various routes. The products of heated cooking oil have toxicity to Drosophila Melanogaster, and maybe display it's bad effect through those genes we founded by gene chip. So, it's necessary to strengthen the study on molecular mechanism of it's toxicity for public health.